Comparison between sonodynamic effect with protoporphyrin IX and hematoporphyrin on sarcoma 180

被引:73
|
作者
Liu, QuanHong [1 ]
Wang, XiaoBing [1 ]
Wang, Pan [1 ]
Xiao, LiNa [1 ]
Hao, Qiao [1 ]
机构
[1] Shaanxi Normal Univ, Coll Life Sci, Xian 710062, Peoples R China
基金
中国国家自然科学基金;
关键词
sonodynamic therapy; anti-tumor effect; protoporphyrin IX; hematoporphyrin; sarcoma; 180;
D O I
10.1007/s00280-006-0413-4
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose The comparison between sonodynamic antitumor effect with protoporphyrin IX (PPIX) and hematoporphyrin (Hp) at a concentration of 5 mg/kg on Sarcoma 180 (S180) cells was studied in vivo, and the potential cell damage mechanism was also investigated. Methods The sonodynamically induced anti-tumor effect of PPIX was studied in mice bearing S180 solid tumors. In order to determine the optimum timing of ultrasound exposure after administration of PPIX, the PPIX concentrations in plasma, skin, muscle and tumor were determined by the fluorescence intensity of tissue extractions with a fluorescence spectrophotometer based on the standard curve. Anti-tumor effects were estimated by measuring the tumor size and the tumor weight. Additionally, the morphological changes of S180 cells were evaluated by transmission electron microscope (TEM) observation immediately after sonodynamic therapy (SDT) treatment. Results A time of 24 h after the intravenous administration of PPIX was chosen as the best time for ultrasound exposure. The antitumor effect induced by PPIX mediated sonodynamic therapy (PPIX-SDT) was in a dose dependent manner when ultrasound intensity was at or above the inertial cavitation threshold (5 W/cm(2)). A significant tumor growth delay was observed both in PPIX mediated sonodynamic therapy and in Hp mediated sonodynamic therapy treatments (Hp-SDT), and the tumor weight inhibition ratios after the synergistic treatments were 42.82 +/- 0.03 and 35.22 +/- 0.03%, respectively, this difference was significant at P < 0.05. While ultrasound alone (5 W/cm2supercript stop) showed a slight tumor growth inhibitory effect compared with the control group, and PPIX or Hp alone showed almost no significant effect. Furthermore, TEM observation indicated cell damage was more serious in PPIX-SDT treatment group than in Hp-SDT treatment group. After sonication, the cell ultra-structure such as cell membrane destruction, mitochondria swelling, chromatin condensation might be important factors that inhibited the tumor growth and even induced cell death. Conclusions The comparative results suggested that PPIX as a sonosensitizer might have more potential cytotoxicity than Hp when irradiated with ultrasound, and the ultra-structural changes may account for cell destruction induced by sonodynamic therapy in our experiment mode.
引用
收藏
页码:671 / 680
页数:10
相关论文
共 50 条
  • [21] Transfer of protoporphyrin IX between cells
    Moan, J
    Ma, LW
    Stokke, T
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2002, 76 (05) : 526 - 529
  • [22] Apoptosis induced by sonodynamic treatment by protoporphyrin IX on MDA-MB-231 cells
    Li, Yixiang
    Wang, Pan
    Zhao, Ping
    Zhu, Sijia
    Wang, Xiaobing
    Liu, Quanhong
    ULTRASONICS, 2012, 52 (04) : 490 - 496
  • [23] APOPTOSIS OF THP-1 MACROPHAGES INDUCED BY PROTOPORPHYRIN IX MEDIATED SONODYNAMIC THERAPY
    Xu Haobo
    Tian Ye
    HEART, 2013, 99 : E79 - E80
  • [24] The photosensitizing effect of the photoproduct of protoporphyrin IX
    Ma, LW
    Bagdonas, S
    Moan, J
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2001, 60 (2-3) : 108 - 113
  • [25] Photodynamic efficiency of protoporphyrin IX: Comparison of endogenous protoporphyrin IX induced by 5-aminolevulinic acid and exogenous porphyrin IX
    Tabata, K
    Ogura, S
    Okura, I
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (06) : 842 - 846
  • [26] Apoptotic effects of sonodynamic therapy with protoporphyrin IX on SAS human tongue carcinoma cell and Xenografts
    Cao, Wenwu
    Zheng, Jinhua
    Lv, Yanhong
    Song, Wei
    Cui, Haidong
    Gao, Zhongxiuzi
    Li, Haixia
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2011, 28 : S20 - S20
  • [27] Protoporphyrin IX-mediated sonodynamic action induces apoptosis of K562 cells
    Su, Xiaomin
    Li, Yixiang
    Wang, Pan
    Wang, Xiaobing
    Liu, Quanhong
    ULTRASONICS, 2014, 54 (01) : 275 - 284
  • [28] Apoptosis of THP-1 macrophages induced by protoporphyrin IX-mediated sonodynamic therapy
    Guo, Shuyuan
    Sun, Xin
    Cheng, Jiali
    Xu, Haobo
    Dan, Juhua
    Shen, Jing
    Zhou, Qi
    Zhang, Yun
    Meng, Lingli
    Cao, Wenwu
    Tian, Ye
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2013, 8 : 2239 - 2246
  • [29] Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells
    Li, Qingsong
    Kang, Jian
    Xiong, Xiaojing
    Liu, Yan
    Cao, Wenwu
    Liu, Ying
    Li, Yongning
    ONCOLOGY LETTERS, 2017, 14 (02) : 2097 - 2102
  • [30] A comparison of protoporphyrin IX and protoporphyrin IX dimethyl ester as a photosensitizer in poorly differentiated human nasopharyngeal carcinoma cells
    Yee, KKL
    Soo, KC
    Bay, BH
    Olivo, M
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2002, 76 (06) : 678 - 682