Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Cannabis sativa

被引：19
作者：
Kitamura, Masashi [1 ,2 ]
Aragane, Masako [3 ]
Nakamura, Kou [3 ]
Watanabe, Kazuhito [4 ]
Sasaki, Yohei [1 ]
机构：
[1] Kanazawa Univ, Grad Sch Med Sci, Div Pharmaceut Sci, Lab Mol Pharmacognosy, Kakuma Machi, Kanazawa, Ishikawa 9201192, Japan
[2] Ishikawa Prefectural Police HQ, Forens Sci Lab, 1-1 Kuratsuki, Kanazawa, Ishikawa 9208553, Japan
[3] Tokyo Metropolitan Inst Publ Hlth, Med Plant Garden, 21-1 Nakajima Cho, Tokyo 1870033, Japan
[4] Hokuriku Univ, Fac Pharmaceut Sci, Ho 3 Kanagawa Machi, Kanazawa, Ishikawa 9201181, Japan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2016年 / 39卷 / 07期
关键词：
Cannabis sativa; isothermal amplification; tetrahydrocannabinolic acid; loop-mediated isothermal amplification; marijuana; hemp; IDENTIFICATION; DNA; MARIJUANA; VIRUS; CONSTITUENTS; EXTRACTS; SYNTHASE; PLANTS; GENE; DRUG;
D O I：
10.1248/bpb.b16-00090
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In many parts of the world, the possession and cultivation of Cannabis sativa L. are restricted by law. As chemical or morphological analyses cannot identify the plant in some cases, a simple yet accurate DNA-based method for identifying C. sativa is desired. We have developed a loop-mediated isothermal amplification (LAMP) assay for the rapid identification of C. sativa. By optimizing the conditions for the LAMP reaction that targets a highly conserved region of tetrahydrocannabinolic acid (THCA) synthase gene, C. sativa was identified within 50 min at 60-66 degrees C. The detection limit was the same as or higher than that of conventional PCR. The LAMP assay detected all 21 specimens of C. sativa, showing high specificity. Using a simple protocol, the identification of C. sativa could be accomplished within 90 min from sample treatment to detection without use of special equipment. A rapid, sensitive, highly specific, and convenient method for detecting and identifying C. sativa has been developed and is applicable to forensic investigations and industrial quality control.
引用
收藏
页码：1144 / 1149
页数：6
相关论文
共 31 条
[11]   A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae) [J].
Hillig, KW ;
Mahlberg, PG .
AMERICAN JOURNAL OF BOTANY, 2004, 91 (06) :966-975
[12]   Evaluating the Quality of Protein from Hemp Seed (Cannabis sativa L.) Products Through the use of the Protein Digestibility-Corrected Amino Acid Score Method [J].
House, James D. ;
Neufeld, Jason ;
Leson, Gero .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (22) :11801-11807
[13]   Species Identification of Cannabis sativa Using Real-Time Quantitative PCR (qPCR) [J].
Johnson, Christopher E. ;
Premasuthan, Amritha ;
Trask, Jessica Satkoski ;
Kanthaswamy, Sree .
JOURNAL OF FORENSIC SCIENCES, 2013, 58 (02) :486-490
[14]   LAMP assay and rapid sample preparation method for on-site detection of flavescence doree phytoplasma in grapevine [J].
Kogovsek, P. ;
Hodgetts, J. ;
Hall, J. ;
Prezelj, N. ;
Nikolic, P. ;
Mehle, N. ;
Lenarcic, R. ;
Rotter, A. ;
Dickinson, M. ;
Boonham, N. ;
Dermastia, M. ;
Ravnikar, M. .
PLANT PATHOLOGY, 2015, 64 (02) :286-296
[15]   DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L. [J].
Kojoma, Mareshige ;
Seki, Hikaru ;
Yoshida, Shigeo ;
Muranaka, Toshiya .
FORENSIC SCIENCE INTERNATIONAL, 2006, 159 (2-3) :132-140
[16]   Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification [J].
Kurosaki, Yohei ;
Takada, Ayato ;
Ebihara, Hideki ;
Grolla, Allen ;
Kamo, Naoki ;
Feldmann, Heinz ;
Kawacka, Yoshihiro ;
Yasuda, Jiro .
JOURNAL OF VIROLOGICAL METHODS, 2007, 141 (01) :78-83
[17]   Detection and identification of cannabis by DNA [J].
Linacre, A ;
Thorpe, J .
FORENSIC SCIENCE INTERNATIONAL, 1998, 91 (01) :71-76
[18]   EXAMINATION OF CYSTOLITHIC HAIRS OF CANNABIS AND OTHER PLANTS BY MEANS OF SCANNING ELECTRON-MICROSCOPE [J].
MITOSINKA, GT ;
THORNTON, JI ;
HAYES, TL .
JOURNAL OF THE FORENSIC SCIENCE SOCIETY, 1972, 12 (03) :521-+
[19]   Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation [J].
Mori, Y ;
Nagamine, K ;
Tomita, N ;
Notomi, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 289 (01) :150-154
[20]   New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification [J].
Mu, Yonglin ;
Zeng, Jiawei ;
Chen, Qianqian ;
Liu, Jia ;
Wang, Lili ;
Yao, Fujia ;
Cui, Meng ;
He, Zhixiang ;
Zhang, Chiyu ;
Xiao, Ming ;
Lan, Ke .
JOURNAL OF VIROLOGICAL METHODS, 2014, 206 :84-88
1234