Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Cannabis sativa

被引：18

作者：

Kitamura, Masashi ^{[1
,2
]}

Aragane, Masako ^{[3
]}

Nakamura, Kou ^{[3
]}

Watanabe, Kazuhito ^{[4
]}

Sasaki, Yohei ^{[1
]}

机构：

[1] Kanazawa Univ, Grad Sch Med Sci, Div Pharmaceut Sci, Lab Mol Pharmacognosy, Kakuma Machi, Kanazawa, Ishikawa 9201192, Japan

[2] Ishikawa Prefectural Police HQ, Forens Sci Lab, 1-1 Kuratsuki, Kanazawa, Ishikawa 9208553, Japan

[3] Tokyo Metropolitan Inst Publ Hlth, Med Plant Garden, 21-1 Nakajima Cho, Tokyo 1870033, Japan

[4] Hokuriku Univ, Fac Pharmaceut Sci, Ho 3 Kanagawa Machi, Kanazawa, Ishikawa 9201181, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2016年 / 39卷 / 07期

关键词：

Cannabis sativa; isothermal amplification; tetrahydrocannabinolic acid; loop-mediated isothermal amplification; marijuana; hemp; IDENTIFICATION; DNA; MARIJUANA; VIRUS; CONSTITUENTS; EXTRACTS; SYNTHASE; PLANTS; GENE; DRUG;

D O I：

10.1248/bpb.b16-00090

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In many parts of the world, the possession and cultivation of Cannabis sativa L. are restricted by law. As chemical or morphological analyses cannot identify the plant in some cases, a simple yet accurate DNA-based method for identifying C. sativa is desired. We have developed a loop-mediated isothermal amplification (LAMP) assay for the rapid identification of C. sativa. By optimizing the conditions for the LAMP reaction that targets a highly conserved region of tetrahydrocannabinolic acid (THCA) synthase gene, C. sativa was identified within 50 min at 60-66 degrees C. The detection limit was the same as or higher than that of conventional PCR. The LAMP assay detected all 21 specimens of C. sativa, showing high specificity. Using a simple protocol, the identification of C. sativa could be accomplished within 90 min from sample treatment to detection without use of special equipment. A rapid, sensitive, highly specific, and convenient method for detecting and identifying C. sativa has been developed and is applicable to forensic investigations and industrial quality control.

引用

页码：1144 / 1149

页数：6

共 31 条

[1] Cannabinoid ester constituents from high-potency Cannabis sativa
Ahmed, Safwat A.
Ross, Samir A.
Slade, Desmond
Radwan, Mohamed M.
Zulfiqar, Fazila
ElSohly, Mahmoud A.
[J]. JOURNAL OF NATURAL PRODUCTS, 2008, 71 (04): : 536 - 542
[2] Identification and quantification of cannabinoids in Cannabis sativa L. plants by high performance liquid chromatography-mass spectrometry
Aizpurua-Olaizola, Oier
Omar, Jone
Navarro, Patricia
Olivares, Maitane
Etxebarria, Nestor
Usobiaga, Aresatz
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (29) : 7549 - 7560
[3] de Meijer EPM, 2003, GENETICS, V163, P335
[4] Debruyne D., 1994, Bulletin on Narcotics, V46, P109
[5] Chemical constituents of marijuana: The complex mixture of natural cannabinoids
ElSohly, MA
Slade, D
[J]. LIFE SCIENCES, 2005, 78 (05) : 539 - 548
[6] The Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Salmonella enterica serovar Typhi
Fan, Fenxia
Du, Pengcheng
Kan, Biao
Yan, Meiying
[J]. PLOS ONE, 2015, 10 (04):
[7] Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction
Fukuta, S
Kato, S
Yoshida, K
Mizukami, Y
Ishida, A
Ueda, J
Kanbe, M
Ishimoto, Y
[J]. JOURNAL OF VIROLOGICAL METHODS, 2003, 112 (1-2) : 35 - 40
[8] Gigliano GS, 1999, J FORENSIC SCI, V44, P475
[9] Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts
Grauwiler, Sandra B.
Scholer, Andre
Drewe, Juergen
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 850 (1-2): : 515 - 522
[10] Pharmacokinetics and pharmacodynamics of cannabinoids
Grotenhermen, F
[J]. CLINICAL PHARMACOKINETICS, 2003, 42 (04) : 327 - 360

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