Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents

被引：35
|
作者：
Nguyen, HK
Fournier, O
Asseline, U
Dupret, D
Thuong, NT
机构：
[1] CNRS, Ctr Biophys Mol, F-45071 Orleans 02, France
[2] Appligene Oncor, F-67402 Illkirch Graffenstaden, France
来源：
NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 06期
关键词：
D O I：
10.1093/nar/27.6.1492
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA(+) along with the chemical modification of duplexes allow the fine adjustment of T-m and the possibility of obtaining several duplex systems with varied isostabilized temperatures, some of which show greater stability than those of natural DNA. This approach could be very useful for DNA sequencing by hybridization.
引用
收藏
页码：1492 / 1498
页数：7
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