Detecting Intestinal Goblet Cells of the Broadgilled Hagfish Eptatretus cirrhatus (Forster, 1801): A Confocal Microscopy Evaluation

被引:31
作者
Alesci, Alessio [1 ]
Pergolizzi, Simona [1 ]
Savoca, Serena [2 ,3 ]
Fumia, Angelo [4 ]
Mangano, Angelica [1 ]
Albano, Marco [1 ]
Messina, Emmanuele [1 ]
Aragona, Marialuisa [5 ]
Lo Cascio, Patrizia [1 ]
Capillo, Gioele [2 ,3 ]
Lauriano, Eugenia Rita [1 ]
机构
[1] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, I-98166 Messina, Italy
[2] Univ Messina, Dept Biomed Dent & Morphol & Funct Imaging, I-98125 Messina, Italy
[3] CNR, Natl Res Council IRBIM, Inst Marine Biol Resources & Biotechnol, I-98164 Messina, Italy
[4] Univ Messina, AOU Policlin G Martino, Dept Clin & Expt Med, Padigl C, I-98124 Messina, Italy
[5] Univ Messina, Dept Vet Sci, I-98168 Messina, Italy
来源
BIOLOGY-BASEL | 2022年 / 11卷 / 09期
关键词
confocal microscopy; Eptatretus cirrhatus; goblet cells; gut; immune system; NITRIC-OXIDE; ANTIMICROBIAL PEPTIDES; DENDRITIC CELLS; INNATE IMMUNITY; NOS ISOFORMS; FISH; EXPRESSION; TELEOST; BASS; EPITHELIUM;
D O I
10.3390/biology11091366
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Simple Summary The intestinal epithelium of fish, similar to mammals, consists mainly of enterocytes and goblet cells. Goblet cells play a key role in the secretion of mucus, which, in addition to promoting the digestion of nutrients, is the first protective barrier against bacteria, viruses, and pathogens. Our study aims to evaluate the presence, localization, and co-localization of 5-HT, TLR2, iNOS, and Piscidin1 in goblet cells of the intestine of Eptatretus cirrhatus. The results obtained by confocal microscopy show, for the first time, the positivity of goblet cells to the antibodies tested, suggesting the involvement of these cells in the intestinal immunity of broadgilled hagfish. The fish intestine operates as a complicated interface between the organism and the environment, providing biological and mechanical protections as a result of a viscous layer of mucus released by goblet cells, which serves as a barrier against bacteria, viruses, and other pathogens, and contributes to the functions of the immune system. Therefore, goblet cells have a role in preserving the health of the body by secreting mucus and acting as sentinels. The ancient jawless fish broadgilled hagfish (Eptatretus cirrhatus, Forster, 1801) has a very basic digestive system because it lacks a stomach. By examining the presence, localization, and co-localization of 5-HT, TLR2, iNOS, and Piscidin1, this study intends to provide insight into the potential immune system contributions arranged by the gut goblet cells of broadgilled hagfish. Our results characterize intestinal goblet cells of broadgilled hagfish, for the first time, with the former antibodies, suggesting the hypothesis of conservation of the roles played by these cells also in primitive vertebrates. Moreover, this study deepens the knowledge about the still little-known immune system of hagfish.
引用
收藏
页数:11
相关论文
共 69 条
[1]   Confocal Characterization of Intestinal Dendritic Cells from Myxines to Teleosts [J].
Alesci, Alessio ;
Capillo, Gioele ;
Fumia, Angelo ;
Messina, Emmanuele ;
Albano, Marco ;
Aragona, Marialuisa ;
Lo Cascio, Patrizia ;
Spano, Nunziacarla ;
Pergolizzi, Simona ;
Lauriano, Eugenia Rita .
BIOLOGY-BASEL, 2022, 11 (07)
[2]   Histological and Chemical Analysis of Heavy Metals in Kidney and Gills of Boops boops: Melanomacrophages Centers and Rodlet Cells as Environmental Biomarkers [J].
Alesci, Alessio ;
Cicero, Nicola ;
Fumia, Angelo ;
Petrarca, Claudia ;
Mangifesta, Rocco ;
Nava, Vincenzo ;
Lo Cascio, Patrizia ;
Gangemi, Sebastiano ;
Di Gioacchino, Mario ;
Lauriano, Eugenia Rita .
TOXICS, 2022, 10 (05)
[3]   Localization of vasoactive intestinal peptide and toll-like receptor 2 immunoreactive cells in endostyle of urochordate Styela plicata (Lesueur, 1823) [J].
Alesci, Alessio ;
Pergolizzi, Simona ;
Lo Cascio, Patrizia ;
Capillo, Gioele ;
Lauriano, Eugenia Rita .
MICROSCOPY RESEARCH AND TECHNIQUE, 2022, 85 (07) :2651-2658
[4]   Relationship between Immune Cells, Depression, Stress, and Psoriasis: Could the Use of Natural Products Be Helpful? [J].
Alesci, Alessio ;
Lauriano, Eugenia Rita ;
Fumia, Angelo ;
Irrera, Natasha ;
Mastrantonio, Enza ;
Vaccaro, Mario ;
Gangemi, Sebastiano ;
Santini, Antonello ;
Cicero, Nicola ;
Pergolizzi, Simona .
MOLECULES, 2022, 27 (06)
[5]   Rodlet cells in kidney of goldfish (Carassius auratus, Linnaeus 1758): A light and confocal microscopy study [J].
Alesci, Alessio ;
Pergolizzi, Simona ;
Capillo, Gioele ;
Lo Cascio, Patrizia ;
Lauriano, Eugenia Rita .
ACTA HISTOCHEMICA, 2022, 124 (03)
[6]   Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization [J].
Alesci, Alessio ;
Pergolizzi, Simona ;
Fumia, Angelo ;
Calabro, Concetta ;
Lo Cascio, Patrizia ;
Lauriano, Eugenia Rita .
ACTA ZOOLOGICA, 2023, 104 (03) :366-379
[7]   Neuronal regeneration: Vertebrates comparative overview and new perspectives for neurodegenerative diseases [J].
Alesci, Alessio ;
Pergolizzi, Simona ;
Lo Cascio, Patrizia ;
Fumia, Angelo ;
Lauriano, Eugenia Rita .
ACTA ZOOLOGICA, 2022, 103 (02) :129-140
[8]   Can nutraceuticals assist treatment and improve covid-19 symptoms? [J].
Alesci, Alessio ;
Aragona, Marialuisa ;
Cicero, Nicola ;
Lauriano, Eugenia Rita .
NATURAL PRODUCT RESEARCH, 2022, 36 (10) :2672-2691
[9]   Expression profile of toll-like receptor 2 mRNA in selected tissues of shark (Chiloscyllium sp.) [J].
Anandhakumar, C. ;
Lavanya, V. ;
Pradheepa, G. ;
Tirumurugaan, K. G. ;
Raj, G. Dhinakar ;
Raja, A. ;
Pazhanivel, N. ;
Balachandran, C. .
FISH & SHELLFISH IMMUNOLOGY, 2012, 33 (05) :1174-1182
[10]   Evolution of the nitric oxide synthase family in vertebrates and novel insights in gill development [J].
Annona, Giovanni ;
Sato, Iori ;
Pascual-Anaya, Juan ;
Osca, David ;
Braasch, Ingo ;
Voss, Randal ;
Stundl, Jan ;
Soukup, Vladimir ;
Ferrara, Allyse ;
Fontenot, Quenton ;
Kuratani, Shigeru ;
Postlethwait, John H. ;
D'Aniello, Salvatore .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2022, 289 (1980)