Characterization, antioxidant and antiglycation properties of polysaccharides extracted from the medicinal halophyte Carpobrotus edulis L

被引:30
作者
Hafsa, Jawhar [1 ]
Hammi, Khaoula Mkadmini [2 ]
Le Cerf, Didier [3 ,4 ]
Limem, Khalifa [1 ]
Majdoub, Hatem [5 ]
Charfeddine, Bassem [1 ]
机构
[1] Univ Sousse, Fac Med Sousse, Dept Biochem, Sousse 4002, Tunisia
[2] Biotechnol Ctr Borj Cedria CBBC, Lab Med & Aromat Plants, BP 901, Hammam Lif 2050, Tunisia
[3] Univ Normandy, Lab Polymers Biopolymers Surfaces PBS, UMR 6270, F-76821 Mont St Aignan, France
[4] Univ Rouen, FR3038, F-76821 Mont St Aignan, France
[5] Univ Monastir, Fac Sci Monastir, Lab Interfaces & Adv Mat, Monastir 5000, Tunisia
关键词
Carpobrotus edulis; Pectic polysaccharides; Ultrasonic extraction; Antioxidant; Antiglycation; GLYCATION END-PRODUCTS; OPTIMIZATION; DYNAMICS; PECTIN; PLANTS; ACID; RSM;
D O I
10.1016/j.ijbiomac.2017.09.046
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, Box-Behnken design was used to optimize the ultrasonic extraction of Carpobrotus edulis polysaccharides (CEP), and the effect of time, extraction temperature and water to material ratio was evaluated. Optimum conditions were 1.77 h, 78.0 degrees C and 33.04 mL/g to improved CEP yield (7.84%), which is in good agreement with the predicted yield 7.77%. Then, the physico-chemical, antioxidant and antiglycation properties of optimized CEP were studied, and the total sugar and galacturonic acid content were 89.7 and 63.2%, respectively. The composition of neutral monosaccharide was arabinose, xylose, rham-nose and mannose in the molar percentage of 71.84,14.80,8.57, and 4.79%, respectively. In addition, (H-1, and C-13) NMR and FTIR analyses confirmed the presence of uronic acids in the free and methyl ester forms with a degree of esterification of 31.27%. Therefore, this finding showed that CEP is a low methoxyl pectic polysaccharide, with an average molecular weight about 65,000 g/mol. Finally, the results indicated that CEP presents strong antioxidant activities in vitro (DPPH, chelating ability and reducing power), and significantly inhibits lipid peroxidation and the formation of fluorescent advanced glycation end products in glucose-BSA system model. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:833 / 842
页数:10
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