Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

被引：12
作者：
De Stasio, G
Gilbert, B
Perfetti, L
Hansen, R
Mercanti, D
Ciotti, MT
Andres, R
White, VE
Perfetti, P
Margaritondo, G
机构：
[1] CNR, Ist Struttura Mat, I-00133 Rome, Italy
[2] Ecole Polytech Fed Lausanne, Inst Phys Appl, PH Ecublens, CH-1015 Lausanne, Switzerland
[3] Univ Roma Tor Vergata, Rome, Italy
[4] Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA
[5] CNR, Ist Neurobiol, I-00137 Rome, Italy
[6] Paul Scherrer Inst, CH-5232 Villigen, Switzerland
[7] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA
[8] Sincrotrone Trieste SCpA, Trieste, Italy
来源：
ANALYTICAL BIOCHEMISTRY | 1999年 / 266卷 / 02期
关键词：
synchrotron spectromicroscopy; microchemical analysis; MEPHISTO; ashing; incineration; trace element;
D O I：
10.1006/abio.1998.2901
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability. (C) 1999 Academic Press.
引用
收藏
页码：174 / 180
页数：7
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