The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research

被引：14

作者：

So, Seongjun ^{[1
]}

Lee, Yeonmi ^{[1
]}

Choi, Jiwan ^{[1
]}

Kang, Seoon ^{[1
]}

Lee, Ji-Yoon ^{[2
]}

Hwang, Julie ^{[1
]}

Shin, Joosung ^{[1
]}

Dutton, James R. ^{[3
]}

Seo, Eul-Ju ^{[4
]}

Lee, Beom Hee ^{[4
]}

Kim, Chong Jai ^{[5
]}

Mitalipov, Shoukhrat ^{[6
]}

Oh, Soo Jin ^{[2
]}

Kang, Eunju ^{[1
,2
]}

机构：

[1] Univ Ulsan, Asan Inst Life Sci, Stem Cell Ctr, Asan Med Ctr,Coll Med, Seoul, South Korea

[2] Univ Ulsan, Asan Inst Life Sci, Dept Convergence Med, Asan Med Ctr,Coll Med, Seoul, South Korea

[3] Univ Minnesota, Stem Cell Inst, Minneapolis, MN USA

[4] Univ Ulsan, Asan Inst Life Sci, Med Genet Ctr, Asan Med Ctr,Coll Med, Seoul, South Korea

[5] Univ Ulsan, Asan Inst Life Sci, Dept Pathol, Asan Med Ctr,Coll Med, Seoul, South Korea

[6] Oregon Hlth & Sci Univ, Ctr Embryon Cell & Gene Therapy, Portland, OR 97201 USA

来源：

PLOS ONE | 2020年 / 15卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

IN-VITRO; ROCK; DIFFERENTIATION; SURVIVAL; CULTURE; PROLIFERATION; GROWTH; LINES;

D O I：

10.1371/journal.pone.0233057

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.

引用

页数：17

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