Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri fusaro

被引：84

作者：

Metcalf, WW

Zhang, JK

Shi, X

Wolfe, RS

机构：

[1] Department of Microbiology, University of Illinois, Urbana

[2] Department of Microbiology, 131 Burrill Hall, University of Illinois, Urbana, IL 61801

[3] Department of Food Nutrition, University of Illinois, Urbana

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 19期

关键词：

D O I：

10.1128/jb.178.19.5797-5802.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gene express phosphoserine aminotransferase activity, verifying the function of this gene.

引用

页码：5797 / 5802

页数：6

共 24 条

[1] IDENTIFICATION, MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF A GENE-CLUSTER ENCODING THE CLASS-II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE, 3-PHOSPHOGLYCERATE KINASE AND A PUTATIVE 2ND GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE OF ESCHERICHIA-COLI [J].