Reversible enhancement of fluorescence in acidic pH driven by tryptophan stabilized copper nanoclusters and its application in bioimaging

被引：14

作者：

Anusuyadevi, Kathiresan ^{[1
]}

Wu, Shu Pao ^{[2
]}

Velmathi, Sivan ^{[1
]}

机构：

[1] Natl Inst Technol, Dept Chem, Organ & Polymer Synth Lab, Tiruchirapalli 620015, India

[2] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2021年 / 421卷

关键词：

pH sensor; Copper nanocluster; Bioimaging; High temperature synthesis; Reversible enhancement; AGGREGATION-INDUCED EMISSION; SILVER NANOCLUSTERS; GOLD NANOCLUSTERS; SENSOR;

D O I：

10.1016/j.jphotochem.2021.113526

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Aqueous soluble tryptophan templated copper nanoclusters (Trp-CuNCs) are synthesized in one step utilizing eco-friendly precursors and employed in pH sensing as pH acts as an indispensable tool in various fields like chemistry, biology, agriculture etc. Intriguingly, Trp-CuNCs manifested reversible enhancement in emission on moving from basic to acidic pH (9.0-3.0) while turn-on emission in acidic medium is uncommon in reported literatures. The observed peculiarity was due to the presence of indole group in tryptophan which caused aggregation induced quenching (AIQ) in basic medium and it was corroborated by Dynamic Light Scattering (DLS). The role of indole group in aggregation was further substantiated by carrying out control experiments. The type of quenching encountered was identified as static quenching by PL-lifetime experiments. To extend the pragmatic applicability of Trp-CuNCs, bioimaging of HeLa cells under different pH and real sample analysis (water and an antacid product) were performed.

引用

页数：7

共 30 条

[1] Opportunities and challenges in energy and electron transfer of nanocluster based hybrid materials and their sensing applications [J].

Bain, Dipankar ;

Maity, Subarna ;

Patra, Amitava .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2019, 21 (11) :5863-5881

[2] The synthesis of switch-off fluorescent water-stable copper nanocluster Hg2+ sensors via a simple one-pot approach by an in situ metal reduction strategy in the presence of a thiolated polymer ligand template [J].

Benavides, Jesus ;

Quijada-Garrido, Isabel ;

Garcia, Olga .

NANOSCALE, 2020, 12 (02) :944-955

[3] One-step green synthetic approach for the preparation of multicolor emitting copper nanoclusters and their applications in chemical species sensing and bioimaging [J].

Bhamore, Jigna R. ;

Jha, Sanjay ;

Mungara, Anil Kumar ;

Singhal, Rakesh Kumar ;

Sonkeshariya, Dhanshri ;

Kailasa, Suresh Kumar .

BIOSENSORS & BIOELECTRONICS, 2016, 80 :243-248

[4]

Cai Z., 2019, CHEM PAP, V74, P1

[5] Green and facile synthesis of polyethyleneimine-protected fluorescent silver nanoclusters for the highly specific biosensing of curcumin [J].

Cai, Zhifeng ;

Wu, Liangliang ;

Xi, Jierui ;

Hao, Erxiao ;

Qi, Kaifei .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2021, 615

[6] Origin of Strong Excitation Wavelength Dependent Fluorescence of Graphene Oxide [J].

Cushing, Scott K. ;

Li, Ming ;

Huang, Fuqiang ;

Wu, Nianqiang .

ACS NANO, 2014, 8 (01) :1002-1013

[7] Quantum Dot-Fluorescent Protein FRET Probes for Sensing Intracellular pH [J].

Dennis, Allison M. ;

Rhee, Won Jong ;

Sotto, David ;

Dublin, Steven N. ;

Bao, Gang .

ACS NANO, 2012, 6 (04) :2917-2924

[8] Dual Site-Controlled and Lysosome-Targeted Intramolecular Charge Transfer-Photoinduced Electron Transfer-Fluorescence Resonance Energy Transfer Fluorescent Probe for Monitoring pH Changes in Living Cells [J].

Dong, Baoli ;

Song, Xuezhen ;

Wang, Chao ;

Kong, Xiuqi ;

Tang, Yonghe ;

Lin, Weiying .

ANALYTICAL CHEMISTRY, 2016, 88 (07) :4085-4091

[9] Blue-Emitting Copper Nanoclusters Synthesized in the Presence of Lysozyme as Candidates for Cell Labeling [J].

Ghosh, Rama ;

Sahoo, Amaresh Kumar ;

Ghosh, Siddhartha Sankar ;

Paul, Anumita ;

Chattopadhyay, Arun .

ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (06) :3822-3828

[10] FRET-based colorimetric and ratiometric sensor for visualizing pH change and application for bioimaging in living cells, bacteria and zebrafish [J].

He, Xiaojun ;

Ding, Feng ;

Xu, Wei ;

Xu, Chuchu ;

Li, Yahui ;

Qian, Yuna ;

Zhao, Shengzhe ;

Chen, Hong ;

Shen, Jianliang .

ANALYTICA CHIMICA ACTA, 2020, 1127 :29-38

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