A microscope configuration for nanometer 3-D movement monitoring accuracy

被引：30

作者：

Beiderman, Yevgeny ^{[2
]}

Amsel, Avigail D. ^{[1
]}

Tzadka, Yaniv ^{[1
]}

Fixler, Dror ^{[1
]}

Mico, Vicente ^{[3
]}

Garcia, Javier ^{[3
]}

Teicher, Mina ^{[2
]}

Zalevsky, Zeev ^{[1
]}

机构：

[1] Bar Ilan Univ, Sch Engn, IL-52900 Ramat Gan, Israel

[2] Bar Ilan Univ, Dept Math, IL-52900 Ramat Gan, Israel

[3] Univ Valencia, Dept Opt, E-46100 Burjassot, Spain

来源：

MICRON | 2011年 / 42卷 / 04期

关键词：

Fourier optics; Speckle; Backscattering; Movement monitoring; DIGITAL HOLOGRAPHIC MICROSCOPY; CARDIOMYOCYTE CONTRACTION; PHASE MICROSCOPY; DYNAMICS; CELLS;

D O I：

10.1016/j.micron.2010.05.020

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50 nm in the transverse direction and of 200 nm in the axial direction was achieved. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：366 / 375

页数：10

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