Single-Polypeptide SERCA-Phospholamban Fused FRET Biosensor for High-throughput Cardiac-specific Drug Discovery

被引:0
|
作者
Kleinboehl, Evan [1 ]
Schaaf, Tory [1 ]
Yuen, Samantha [1 ]
Roelike, Lauren N. [1 ]
Svensson, Bengt [1 ]
Thompson, Andrew R. [1 ]
Cornea, Razvan L. [1 ]
Thomas, David D. [1 ]
机构
[1] Univ Minnesota, Biochem Mol Biol & Biophys, Minneapolis, MN USA
关键词
D O I
暂无
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
1539-Pos
引用
收藏
页码:314A / 315A
页数:2
相关论文
共 14 条
  • [1] FRET-Based Probe for High-Throughput DNA Intercalator Drug Discovery and In Vivo Imaging
    Murade, Chandrashekhar U.
    Chaudhuri, Samata
    Nabti, Ibtissem
    Fahs, Hala
    Refai, Fatima S. M.
    Xie, Xin
    Pearson, Yanthe E.
    Gunsalus, Kristin C.
    Shubeita, George T.
    ACS SENSORS, 2021, 6 (06) : 2233 - 2240
  • [2] High-Throughput FRET Assays for cAMP Using the Epac1-Based Biosensor H188 Enable GPCR Agonist Drug Discovery
    Holz, George G.
    Leech, Colin A.
    Chepurny, Oleg G.
    JOURNAL OF GENERAL PHYSIOLOGY, 2017, 149 (09): : 6A - 6A
  • [3] Quantitative FRET technology for SUMOylation cascade and high-throughput screening assay for SUMOylation inhibitor in cancer drug discovery
    Liao, Jiayu
    Wiryawan, Hilda
    Li, Yang
    Song, Yang
    Liu, Yan
    You, Jiacong
    Jiang, Ling
    Malik, Harbani Kaur
    Saavedra, Amanda N.
    Qu, Sophie
    CANCER RESEARCH, 2014, 74 (23)
  • [4] Quantitative FRET technology for SUMOylation cascade and high-throughput screening assay for SUMOylation inhibitor in cancer drug discovery
    Liao, Jiayu
    Wiryawan, Hilda
    Song, Yang
    Liu, Yan
    Jiang, Ling
    Malik, Harbani Kaur
    Saaredra, Amanda N.
    MOLECULAR CANCER THERAPEUTICS, 2013, 12 (05)
  • [5] Discovery of SERCA2a/PLB Activators and Inhibitors by Structure-Based High-throughput Screening using Live Cell FRET Biosensors
    Stroik, Daniel R.
    Yuen, Samantha L.
    Janicek, Kevyn A.
    Schaaf, Tory M.
    Cornea, Razvan L.
    Thomas, David D.
    BIOPHYSICAL JOURNAL, 2018, 114 (03) : 147A - 147A
  • [6] Live-Cell Cardiac-Specific High-Throughput Screening Platform for Drug-Like Molecules That Enhance Ca2+ Transport
    Schaaf, Tory M.
    Kleinboehl, Evan
    Yuen, Samantha L.
    Roelike, Lauren N.
    Svensson, Bengt
    Thompson, Andrew R.
    Cornea, Razvan L.
    Thomas, David D.
    CELLS, 2020, 9 (05)
  • [7] FRET-based High-throughput Screening Biosensor using the Cardiac Ryanodine Receptor (RyR2) N-terminal "ABC'' Domain
    Zhang, Jingyan
    Rebbeck, Robyn T.
    Thomas, David D.
    Petegem, Filip V.
    Cornea, Razvan L.
    BIOPHYSICAL JOURNAL, 2020, 118 (03) : 173A - 174A
  • [8] Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators
    Osha Roopnarine
    Samantha L. Yuen
    Andrew R. Thompson
    Lauren N. Roelike
    Robyn T. Rebbeck
    Philip A. Bidwell
    Courtney C. Aldrich
    Razvan L. Cornea
    David D. Thomas
    Scientific Reports, 13
  • [9] Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators
    Roopnarine, Osha
    Yuen, Samantha L.
    Thompson, Andrew R.
    Roelike, Lauren N.
    Rebbeck, Robyn T.
    Bidwell, Philip A.
    Aldrich, Courtney C.
    Cornea, Razvan L.
    Thomas, David D.
    SCIENTIFIC REPORTS, 2023, 13 (01)
  • [10] Treatment-Specific Immune Phenotypes Identified by nELISA High-Throughput Proteomics Reveal Actionable Insights for Drug Discovery
    Robichaud, Nathaniel
    Ongo, Grant
    Teahulos, Ivan
    Rho, Woojong
    Dagher, Milad
    CANCER IMMUNOLOGY RESEARCH, 2023, 11 (12)