Improvement in the quality of hematopoietic prostaglandin D synthase crystals in a microgravity environment

被引：21

作者：

Tanaka, Hiroaki

Tsurumura, Toshiharu ^{[1
]}

Aritake, Kosuke ^{[1
]}

Furubayashi, Naoki

Takahashi, Sachiko

Yamanaka, Mari

Hirota, Erika

Sano, Satoshi ^{[2
]}

Sato, Masaru ^{[2
]}

Kobayashi, Tomoyuki ^{[2
]}

Tanaka, Tetsuo ^{[2
]}

Inaka, Koji

Urade, Yoshihiro ^{[1
]}

机构：

[1] Osaka Biosci Inst, Osaka, Japan

[2] Japan Aerosp Explorat Agcy, Tokyo, Japan

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2011年 / 18卷

关键词：

prostaglandin D synthase; hematopoietic; H-PGDS; crystal; microgravity; space experiment; anti-inflammation; anti-allergy; JAXA; counter-diffusion; PROTEIN CRYSTAL; DIFFRACTION DATA; DEPLETION ZONE; CRYSTALLIZATION; GROWTH; COUNTERDIFFUSION; LYSOZYME; IMPURITY;

D O I：

10.1107/S0909049510037076

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Human hematopoietic prostaglandin synthase, one of the better therapeutic target enzymes for allergy and inflammation, was crystallized with 22 inhibitors and in three inhibitor-free conditions in microgravity. Most of the space-grown crystals showed better X-ray diffraction patterns than the terrestrially grown ones, indicating the advantage of a microgravity environment on protein crystallization, especially in the case of this protein.

引用

页码：88 / 91

页数：4

共 21 条

[1] Structural and functional characterization of HQL-79, an orally selective inhibitor of human hematopoietic prostaglandin D synthase [J].