Stem Cell Regulation by Arabidopsis WOX Genes

被引：154

作者：

Dolzblasz, Alicja ^{[1
,4
]}

Nardmann, Judith ^{[2
]}

Clerici, Elena ^{[3
]}

Causier, Barry ^{[3
]}

van der Graaff, Eric ^{[1
,5
]}

Chen, Jinhui ^{[1
,6
]}

Davies, Brendan ^{[3
]}

Werr, Wolfgang ^{[2
]}

Laux, Thomas ^{[1
]}

机构：

[1] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, Fac Biol, D-79104 Freiburg, Germany

[2] Univ Cologne, Bioctr Cologne, Inst Dev Biol, Zulpicher St 47b, D-50674 Cologne, Germany

[3] Univ Leeds, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England

[4] Univ Wroclaw, Fac Biol Sci, Inst Expt Biol, Dept Plant Dev Biol, PL-50328 Wroclaw, Poland

[5] Univ Copenhagen, Copenhagen Plant Sci Ctr, Dept Plant & Environm Sci, DK-1871 Taastrup, Denmark

[6] Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Jiangsu, Peoples R China

来源：

MOLECULAR PLANT | 2016年 / 9卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis thaliana; shoot meristem; stem cells; WOX genes; WUSCHEL; LEAF BLADE OUTGROWTH; SHOOT MERISTEM; TRANSCRIPTION FACTOR; OVULE DEVELOPMENT; WUSCHEL; PLANTS; EXPRESSION; REPRESSION; EVOLUTION; TOPLESS;

D O I：

10.1016/j.molp.2016.04.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene amplification followed by functional diversification is a major force in evolution. A typical example of this is seen in the WUSCHEL-RELATED HOMEOBOX (WOX) gene family, named after the Arabidopsis stem cell regulator WUSCHEL. Here we analyze functional divergence in the WOX gene family. Members of the WUS clade, except the cambiumstem cell regulator WOX4, can substitute for WUS function in shoot and floral stem cell maintenance to different degrees. Stem cell function of WUS requires a canonical WUS-box, essential for interaction with TPL/TPR co-repressors, whereas the repressive EAR domain is dispensable and the acidic domain seems only to be required for female fertility. In contrast to the WUS clade, members of the ancient WOX13 and the WOX9 clades cannot support stem cell maintenance. Although the homeodomains are interchangeable between WUS and WOX9 clade members, a WUS-compatible homeodomain together with canonical WUS-box is not sufficient for stem cell maintenance. Our results suggest that WOX function in shoot and floral meristems of Arabidopsis is restricted to the modern WUS clade, suggesting that stem cell control is a derived function. Yet undiscovered functional domains in addition to the homeodomain and the WUS-box are necessary for this function.

引用

页码：1028 / 1039

页数：12

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