Genetic diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) revealed by analysis of the cap gene (ORF-2)

被引：32
作者：
Gomes de Castro, A. M. Martins
Cortez, A.
Heinemann, M. B.
Brandao, P. E.
Richtzenhain, L. J.
机构：
[1] Univ Sao Paulo, Fac Med Vet Zootecn, Dept Med Vet Prevent Saude, Sao Paulo, SP, Brazil
[2] Fac Jaguariuna, Curso Med Vet, Jaguariuna, SP, Brazil
[3] Univ Fed Minas Gerais, Escola Vet, Minas Gerais, Brazil
[4] Circovirus Res Grp, Sao Paulo, Brazil
来源：
ARCHIVES OF VIROLOGY | 2007年 / 152卷 / 08期
基金：
巴西圣保罗研究基金会;
关键词：
D O I：
10.1007/s00705-007-0976-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Porcine circovirus 2 (PCV-2) is associated with a broad range of syndromes. In this study, 19 of 870 samples from pigs from different Brazilian states were found to be positive for PCV-2 by polymerase chain reaction (PCR). A fragment of 700 nt of the cap gene (ORF-2) from the 19 PCV-2-positive samples were sequenced using three pairs of primers (Fa/Ra, Fb/Rb and Fc/Rc). Maximum parsimony genealogy with a heuristic algorithm using the 19 field strain studied here, 21 sequences from GenBank and PCV-1 as an out-group showed the existence of two major clusters (A and B) and the Brazilian strains segregating in both of them. PCV-2 was found in pigs with various clinical signs. No association between clusters of PCV-2 and different states or clinical signs were observed, demonstrating that the exact role of PCV-2 in porcine circovirus diseases (PCVD) in Brazil still needs to be clarified. These results contribute to the molecular characterization of PCV-2, which serve as a basis for the epimiology of PCV-2 infection.
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收藏
页码：1435 / 1445
页数：11
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