First genome report and analysis of chicken H7N9 influenza viruses with poly-basic amino acids insertion in the hemagglutinin cleavage site

被引：15

作者：

Chen, Jidang ^{[1
,2
]}

Zhang, Jipei ^{[1
]}

Zhu, Wanjun ^{[2
]}

Zhang, Yishan ^{[1
]}

Tan, Hualong ^{[1
]}

Liu, Minfang ^{[1
]}

Cai, Mingsheng ^{[3
]}

Shen, Jiaren ^{[4
]}

Hinh Ly ^{[2
]}

Chen, Jianhong ^{[1
]}

机构：

[1] Foshan Univ, Sch Life Sci & Engn, Foshan, Peoples R China

[2] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, Minneapolis, MN 55455 USA

[3] Guangzhou Med Univ, Sch Basic Med Sci, Sinofrench Hoffmann Inst, Dept Pathogen Biol & Immunol, Guangzhou, Guangdong, Peoples R China

[4] Shanghai Municipal Ctr Dis Control & Prevent SCDC, Shanghai, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

A(H7N9) VIRUS; A H7N9; GUANGDONG PROVINCE; MOLECULAR-BASIS; H5N1; PROTEIN; VIRULENCE; OUTBREAK; POULTRY; CHINA;

D O I：

10.1038/s41598-017-10605-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the full-length sequence of two chicken source influenza A (H7N9) viruses found in Guangdong live poultry market (LPM) during the most recent wave of human infections (from October 2016 to the present time). These viruses carry insertion of poly-basic amino acids (KGKRTAR/G) at the protease cleavage site of the HA protein, which were previously found in the highly pathogenic (HP) human influenza A (H7N9) [IAV(H7N9)] strains. Phylogenetic analysis of these two novel avian influenza viruses (AIVs) suggested that their genomes reassorted between the Yangtze River Delta (YRD) and Pearl River Delta (PRD) clades. Molecular clock analysis indicated that they emerged several months before the HP human strains. Collectively, our results suggest that IAV(H7N9) viruses evolve in chickens through antigenic drift to include a signature HP sequence in the HA gene, which highlights challenges in risk assessment and public health management of IAV(H7N9) infections at the human-animal interface.

引用

页数：10

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