The influence of ecto-nucleotidases on Leishmania amazonensis infection and immune response in C57B/6 mice

被引:44
作者
de Souza, Miriam Conceicao [1 ]
de Assis, Elisangela Aparecida [1 ]
Gomes, Rodrigo Saar [1 ]
Marques da Silva, Eduardo de Almeida [1 ]
Melo, Maria Norma [2 ]
Rangel Fietto, Juliana Lopes [3 ]
Crocco Afonso, Luis Carlos [1 ]
机构
[1] Univ Fed Ouro Preto, Lab Imunoparasitol, Dept Ciencias Biol, ICEB NUPEB, BR-35400000 Ouro Preto, MG, Brazil
[2] Univ Fed Minas Gerais, Dept Parasitol, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Vicosa, Dept Bioquim & Biol Mol, BR-36570000 Vicosa, MG, Brazil
关键词
Leishmania amazonensis; e-NTPDase; Adenosine; Macrophage; Chemokines; Virulence; TRIPHOSPHATE DIPHOSPHOHYDROLASE ACTIVITIES; TRYPANOSOMA-CRUZI; ATPASE ACTIVITY; T-CELLS; BIOCHEMICAL-CHARACTERIZATION; HOST; VIRULENCE; ADENOSINE; METACYCLOGENESIS; SUSCEPTIBILITY;
D O I
10.1016/j.actatropica.2010.04.007
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Previous results from our laboratory and from the literature have implicated the expression of ecto-nucleotidases in the establishment of Leishmania infection. In the present study we evaluated the correlation between ecto-nucleotidasic activity and the infectivity of L. amazonensis promastigotes that were kept in culture for short or extended numbers of passages, a condition that is known to decrease parasite infectivity. We also analyzed the immune response associated with the infection by these parasites. As expected, we found that long-term cultured parasites induce the development of smaller lesions than the short-term cultured counterparts. Interestingly, long-term cultured parasites presented reduced ecto-nucleotidasic activity. In addition, cells recovered from animals infected with long-term cultured parasites produced higher amounts of IFN-gamma and have smaller parasite load, after 8 weeks of infection. Furthermore, after 1 week of infection, there is increased expression of the chemokine CCL2 mRNA in animals infected with short-term cultured parasites. Finally, infection of peritoneal macrophages by these parasites also shows marked differences. Thus, while short-term cultured parasites are able to infect a greater proportion of macrophages, cells infected by long-term cultured parasites express higher amounts of CXCL10 mRNA, which may activate these cells to kill the parasites. We suggest that the enzymes involved in metabolism of extracellular nucleotides may have an important role in infection by L. amazonensis, by acting directly in its adhesion to target cells and by modulating host cell chemokine production. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:262 / 269
页数:8
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