Rapid detection of Colletotrichum gloeosporioides in infected strawberry plants using loop-mediated isothermal amplification

被引：19

作者：

Katoh, Hiroshi ^{[1
]}

Fukuda, Takashi ^{[2
]}

Nishigawa, Hisashi ^{[3
]}

Natsuaki, Tomohide ^{[3
]}

机构：

[1] Utsunomiya Univ, Ctr Biosci Res & Educ, Mine Machi 350, Utsunomiya, Tochigi 3218505, Japan

[2] Tochigi Prefectural Agr Expt Stn, Kawaraya Cho 1080, Utsunomiya, Tochigi 3200002, Japan

[3] Utsunomiya Univ, Fac Agr, Mine Machi 350, Utsunomiya, Tochigi 3218505, Japan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2016年 / 82卷 / 04期

关键词：

Strawberry anthracnose; Early diagnosis; Colletotrichum gloeosporioides; Colletotrichum acutatum; Colletotrichum aenigma; Colletotrichum siamense; SENSITIVE DETECTION; VIRUS; ASSAY; IDENTIFICATION; GENE; SOIL; PCR; DNA;

D O I：

10.1007/s10327-016-0665-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We developed a loop-mediated isothermal amplification (LAMP) assay for detecting Colletotrichum gloeosporioides, which causes anthracnose, in strawberry plants. Primer sets targeted the region from the gene encoding 5.8S ribosomal RNA to internal transcribed spacer 2, which is highly conserved in 25 Colletotrichum species in GenBank. Our results indicated that the LAMP assay, which uses template DNA prepared using microwave irradiation, is a simple and sensitive tool for detecting C. gloeosporioides in infected strawberry plants. Further, our results showed that the LAMP assay could differentiate Colletotrichum species such as C. acutatum, C. aenigma, and C. siamense.

引用

页码：190 / 198

页数：9

共 43 条

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