Fast separation of enantiomers by capillary electrophoresis using a combination of two capillaries with different internal diameters

被引：7

作者：

Sebestova, Andrea ^{[1
]}

Petr, Jan ^{[1
]}

机构：

[1] Palacky Univ Olomouc, Dept Analyt Chem, Reg Ctr Adv Technol & Mat, Fac Sci, Olomouc, Czech Republic

来源：

ELECTROPHORESIS | 2017年 / 38卷 / 24期

关键词：

Capillary electrophoresis; Chiral separation; Coupled capillaries; Cyclofructan; High-speed separation; SHORT-END INJECTION; CHIRAL STATIONARY PHASES; LIQUID-CHROMATOGRAPHY; CYCLOFRUCTAN; 6; SELECTOR; CYCLODEXTRIN; SYSTEMS; SINGLE;

D O I：

10.1002/elps.201700213

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of capillaries with different internal diameters was used to accelerate the separation of enantiomers in capillary electrophoresis. Separation of R,S-1,1-binaphthalene-2,2-diyl hydrogen phosphate using isopropyl derivative of cyclofructan 6 was studied as a model system. The best separation conditions included 500mM sodium borate pH 9.5 with 60mM concentration of the chiral selector. Separation lasted approx. 1.5min using the combination of 50 and 100m id capillaries of 9.7cm and 22.9cm, respectively. It allowed approx. 12-fold acceleration in comparison to the traditional long-end separation mainly due to the higher electroosmotic flow generated in the connected capillaries.

引用

页码：3124 / 3129

页数：6

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