Microbial cell lysis and nucleic acid extraction via nanofluidic channel

被引：4

作者：

Kant, Krishna ^{[1
]}

Yoo, Jeongha ^{[2
]}

Amos, Steven ^{[2
]}

Erkelens, Mason ^{[2
]}

Priest, Craig ^{[3
]}

Shapter, Joe G. ^{[1
]}

Losic, Dusan ^{[2
]}

机构：

[1] Flinders Univ S Australia, Sch Chem & Phys Sci, Adelaide, SA 5042, Australia

[2] Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia

[3] Univ S Australia, Ian Wark Res Inst, Adelaide, SA 5095, Australia

来源：

RSC ADVANCES | 2015年 / 5卷 / 30期

基金：

澳大利亚研究理事会;

关键词：

SINGLE; DNA;

D O I：

10.1039/c5ra01336d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 mu m (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.

引用

页码：23886 / 23891

页数：6

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