Microbial cell lysis and nucleic acid extraction via nanofluidic channel

被引：4

作者：

Kant, Krishna ^{[1
]}

Yoo, Jeongha ^{[2
]}

Amos, Steven ^{[2
]}

Erkelens, Mason ^{[2
]}

Priest, Craig ^{[3
]}

Shapter, Joe G. ^{[1
]}

Losic, Dusan ^{[2
]}

机构：

[1] Flinders Univ S Australia, Sch Chem & Phys Sci, Adelaide, SA 5042, Australia

[2] Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia

[3] Univ S Australia, Ian Wark Res Inst, Adelaide, SA 5095, Australia

来源：

RSC ADVANCES | 2015年 / 5卷 / 30期

基金：

澳大利亚研究理事会;

关键词：

SINGLE; DNA;

D O I：

10.1039/c5ra01336d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 mu m (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.

引用

页码：23886 / 23891

页数：6

共 28 条

[1] Nanofluidic devices and their applications
Abgrall, Patirick
Nguyen, Nam Trung
[J]. ANALYTICAL CHEMISTRY, 2008, 80 (07) : 2326 - 2341
[2] Microtechnologies and nanotechnologies for single-cell analysis
Andersson, H
van den Berg, A
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2004, 15 (01) : 44 - 49
[3] Fabrication of single nanofluidic channels in poly(methylmethacrylate) films via focused-ion beam milling for use as molecular gates
Cannon, DM
Flachsbart, BR
Shannon, MA
Sweedler, JV
Bohn, PW
[J]. APPLIED PHYSICS LETTERS, 2004, 85 (07) : 1241 - 1243
[4] Desjardins P. R., 2011, CURRENT PROTOCOLS MO
[5] Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching
Dorfman, Kevin D.
King, Scott B.
Olson, Daniel W.
Thomas, Joel D. P.
Tree, Douglas R.
[J]. CHEMICAL REVIEWS, 2013, 113 (04) : 2584 - 2667
[6] GLASEL JA, 1995, BIOTECHNIQUES, V18, P62
[7] Nanoimprint lithography: Methods and material requirements
Guo, L. Jay
[J]. ADVANCED MATERIALS, 2007, 19 (04) : 495 - 513
[8] Fabrication of 1D nanofluidic channels on glass substrate by wet etching and room-temperature bonding
He, Qiaohong
Chen, Shuang
Su, Yuan
Fang, Qun
Chen, Hengwu
[J]. ANALYTICA CHIMICA ACTA, 2008, 628 (01) : 1 - 8
[9] Fabrication and interfacing of nanochannel devices for single-molecule studies
Hoang, H. T.
Segers-Nolten, I. M.
Berenschot, J. W.
de Boer, M. J.
Tas, N. R.
Haneveld, J.
Elwenspoek, M. C.
[J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2009, 19 (06)
[10] Micro- and nanofluidic systems for high-throughput biological screening
Hong, Jongin
Edel, Joshua B.
deMello, Andrew J.
[J]. DRUG DISCOVERY TODAY, 2009, 14 (3-4) : 134 - 146

← 1 2 3 →