A fluorescence assay for microRNA let-7a by a double-stranded DNA modified gold nanoparticle nanoprobe combined with graphene oxide

被引：21

作者：

Gao, Yuanyuan ^{[1
]}

Tian, Jingjing ^{[1
]}

Zhang, Xing ^{[1
]}

Qiao, Bin ^{[2
,3
,4
]}

Cao, Yang ^{[5
]}

Wang, Xiaohong ^{[1
]}

Wu, Qiang ^{[2
,3
,4
]}

机构：

[1] Hainan Univ, Coll Mat Sci & Engn, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China

[2] Hainan Med Univ, Key Lab Emergency & Trauma, Minist Educ, Haikou 571199, Hainan, Peoples R China

[3] Hainan Med Univ, Res Unit Isl Emergency Med, Chinese Acad Med Sci, Haikou 571199, Hainan, Peoples R China

[4] Hainan Med Univ, Sch Trop Med & Lab Med, Haikou 571199, Hainan, Peoples R China

[5] Qiongtai Normal Univ, Haikou 571127, Hainan, Peoples R China

来源：

ANALYST | 2020年 / 145卷 / 04期

基金：

中国国家自然科学基金;

关键词：

REAL-TIME PCR; ELECTROCHEMICAL DETECTION; AMPLIFICATION; EXPRESSION; BIOSENSOR; DIAGNOSIS; CANCER;

D O I：

10.1039/c9an02274k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Graphene oxide (GO) is capable of quenching fluorescence in bound fuel single stranded DNA (ssDNA) labeled with dye. In the presence of target microRNA let-7a, two cascaded toehold-mediated strand displacement reactions (TSDRs) were triggered, thereby desorbing substantial pi-pi stacking adsorbed fuel DNA from GO through recycling amplification, simultaneously accompanied with the restoration of dye fluorescence. By coupling dsDNA-AuNPs with the GO nanosheet, the proposed strategy achieved a non-enzymatic sensitive assay with a low detection limit of 3.9 pM. Hence, the proposed approach has great potential for applications in early cancer diagnosis and clinical analysis.

引用

页码：1190 / 1194

页数：5

共 43 条

[21] Evaluation of miR-506 and miR-4316 expression in early and non-invasive diagnosis of colorectal cancer [J].