Two phase partitioning and collagen hydrolysis of bromelain from pineapple peel Nang Lae cultivar

Extraction of bromelain from pineapple peel (Nang Lae cultv) using aqueous two phase system (ATPS) was optimized Some biochemical properties including collagen hydrolysis were also investigated Bromelain predominantly partitioned to the polyethylene glycol (PEG)-rich phase The highest enzyme activity recovery (113 54%) and purification fold (2 23) were presented in the top phase of 15% PEG2000-14% MgSO4 Protein pattern and activity staining showed the molecular weight (MW) of bromelain to be about 29 kDa The extracted bromelain showed the highest relative activity at pH 7 0 and 55 C Its activity was decreased continuously by increasing NaCl concentration (up to 1 5% (w/v)) The bromelain extract was applied to hydrolyze the skin collagen of beef and giant catfish (0-03 units) The beta alpha(1) alpha(2) of giant catfish skin collagen extensively degraded into small peptides when treated with 002 units of the bromelain extract Bovine collagen was hydrolyzed using higher bromelain up to 018 units This study showed the ATPS can be employed to partially purify bromelain from Nang Lae pineapple peel and the enzyme effectively hydrolyzed the collagens (C) 2010 Elsevier B V All rights reserved