Cloning, molecular characterization, and expression of an endo-polygalacturonase-encoding gene from Saccharomyces cerevisiae IM1-8b

被引：41

作者：

Blanco, P ^{[1
]}

Sieiro, C ^{[1
]}

Reboredo, NM ^{[1
]}

Villa, TG ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Pharm, Dept Microbiol, Santiago De Compostela 15706, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 164卷 / 02期

关键词：

Saccharomyces cerevisiae; polygalacturonase; polygalacturonase-encoding gene; protein sequence; over-expression;

D O I：

10.1016/S0378-1097(98)00220-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A structural polygalacturonase-encoding gene (PGU1) from Saccharomyces cerevisiae IM1-8b was cloned and sequenced. The predicted protein comprises 361 amino acids, with a signal peptide between residues 1 and 18 and two potential glycosylation points in residues 318 and 330. The putative active site is a conserved histidine in position 222. This polygalacturonase showed 54% homology with the fungal ones and only 24% homology with their plant and bacterial counterparts. The gene is present in a single gene copy per haploid genome and it is detected in all strains, regardless of their phenotype. The expression of PGU1 gene in several strains of S. cerevisiae revealed that the polygalacturonase activity depended on the plasmid used and also on the genetic background of each strain but in all cases the enzymatic activity increased. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：249 / 255

页数：7

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