A new set of versatile vectors for the heterologous expression of foreign genes using the baculovirus system

被引：15
|
作者：
Lenhard, T [1 ]
Maul, G [1 ]
Haase, W [1 ]
Reilander, H [1 ]
机构：
[1] MAX PLANCK INST BIOPHYS,ABT MOLEK MEMBRANBIOL,D-60528 FRANKFURT,GERMANY
来源：
GENE | 1996年 / 169卷 / 02期
关键词：
heterologous expression; immunological detection; purification; melittin; peptide tag;
D O I：
10.1016/0378-1119(95)00831-4
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A new set of versatile advanced baculovirus (BV) vectors for the production of fused proteins in insect cells, under the control of the strong polyhedrin promoter of the Autographa california nuclear polyhedrosis virus (AcMNPV), has been constructed. The vectors contain peptide tags which allow immunological detection, as well as purification of recombinant protein produced via the BV expression system.
引用
收藏
页码：187 / 190
页数：4
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