Dexamethasone regulates the actions of endogenous insulin-like growth factor-II during myogenic differentiation

被引：11
作者：
Yoshiko, Y [1 ]
Hirao, K [1 ]
Maeda, N [1 ]
机构：
[1] Hiroshima Univ, Sch Dent, Dept Oral Anat, Minami Ku, Hiroshima 7348553, Japan
来源：
LIFE SCIENCES | 1998年 / 63卷 / 02期
关键词：
C2C12; myoblasts; dexamethasone; IGF-II; RU-38486;
D O I：
10.1016/S0024-3205(98)00242-2
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The effect of dexamethasone (DEX) on the action of endogenous insulin-like growth factor (IGF)-II during myogenic differentiation was investigated by culturing C2C12 mouse myogenic cells in serum-free medium. DEX treatment maintained a high level of creatine kinase (CK) activity, and caused an increase in the number of nuclei per cell, hypertrophy and IGF-II mRNA accumulation in the cells. These effects were abrogated by the glucocorticoid receptor antagonist RU-38486. An anti-IGF-II monoclonal antibody neutralized DEX-dependent CK activity. Thus, we conclude that DEX increases the level of IGF-II mRNA in C2C12 cells, and that DEX may assist myogenic differentiation via, at least in part, its promotive action on IGF-II gene expression.
引用
收藏
页码：77 / 85
页数：9
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