Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems

被引：59

作者：

Johansson, Hans-Olof ^{[1
]}

Magaldi, Flavio Musa ^{[1
]}

Feitosa, Eloi ^{[2
]}

Pessoa, Adalberto, Jr. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Pharmaceut Technol & Biochem, BR-05508000 Sao Paulo, Brazil

[2] Sao Paulo State Univ, Dept Phys, Sao Jose Do Rio Preto, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1178卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

hemoglobin; lysozyme; glucose-6-phospate dehydrogenase; poly(ethylene glycol); sodium poly(acrylic acid); aqueous two-phase system; liquid - liquid extraction;

D O I：

10.1016/j.chroma.2007.11.071

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The partition of hemoglobin, lysozyme and glucose-6-phospate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na2SO4, pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：145 / 153

页数：9

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