Vancomycin bound biogenic gold nanoparticles: A different perspective for development of anti VRSA agents

被引：105
作者：
Fayaz, A. Mohammed [1 ]
Girilal, M. [1 ]
Mandy, Saba A. [1 ]
Somsundar, S. S. [1 ]
Venkatesan, R. [2 ]
Kalaichelvan, P. T. [1 ]
机构：
[1] Univ Madras, Ctr Adv Studies Bot, Madras 600025, Tamil Nadu, India
[2] Natl Inst Ocean Technol, Madras 600100, Tamil Nadu, India
来源：
PROCESS BIOCHEMISTRY | 2011年 / 46卷 / 03期
关键词：
Biological synthesis; Gold nanoparticles; Trichoderma viride; Vancomycin; SILVER NANOPARTICLES;
D O I：
10.1016/j.procbio.2010.11.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The alarming growth of vancomycin resistant Staphylococcus aureus (VRSA) is the main driving force for the development of a new methodology to restore the antibacterial activity against VRSA. In the present study, gold nanoparticles were biologically synthesized using the non pathogenic fungus Trichoderma viride at room temperature. The extremely stable gold nanoparticles were characterized by using UV-vis spectrophotometer. FTIR, TEM. EDX and XRD. The nanoparticles exhibit maximum absorbance at 520 rim in the UV spectrum. The functional group of protein molecule was identified using FTIR. The presence of elemental gold was confirmed by EDX analysis. The TEM micrograph showed the presence of vancomycin bound gold nanoparticles (VBGNP) in abundance on the cell wall surface of VRSA. The antibacterial mechanism may be due to VBGNP binding non-specifically to transpeptidases, instead of terminal peptidases of the glycopeptidyl precursors on the cell surface of test strain. The VBGNP showed significant antibacterial activity against Escherichia coli strain. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：636 / 641
页数：6
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