Enzymatic enantioselective C-C-bond formation in microreactors

被引：42

作者：

Koch, K. ^{[1
]}

van den Berg, R. J. F. ^{[1
]}

Nieuwland, P. J. ^{[1
]}

Wijtmans, R. ^{[1
]}

Schoemaker, H. E. ^{[2
]}

van Hest, J. C. M. ^{[1
]}

Rutjes, F. P. J. T. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 ED Nijmegen, Netherlands

[2] DSM Pharmaceut Prod Adv Synth Catalysis & Dev, NL-6160 MD Geleen, Netherlands

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2008年 / 99卷 / 04期

关键词：

microreactors; screening; enzyme catalysis; lyases; crude lysates;

D O I：

10.1002/bit.21649

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed.

引用

页码：1028 / 1033

页数：6

共 20 条

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