Application of the hsp65 PRA method for the rapid identification of mycobacteria isolated from clinical samples in Belgium

被引：15
作者：
Martin, Anandi
Uwizeye, Cecile
Fissette, Krista
De Rijk, Pim
Palomino, Juan Carlos
Leao, Sylvia
Portaels, Francoise
机构：
[1] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium
[2] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 71卷 / 01期
关键词：
mycobacteria; PRA; identification;
D O I：
10.1016/j.mimet.2007.07.008
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Biochemical identification of mycobacteria is slow and many times fail to produce correct results. We compared PCR-restriction fragment length polymorphism analysis (PRA) of hsp65 and biochemical methods for the identification of mycobacteria from human samples in Belgium. PRA was found useful in the identification of mycobacteria and simple to implement as a quick method in the laboratory. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：39 / 43
页数：5
相关论文
共 45 条
[1]   Heterogeneity and clonality among isolates of Mycobacterium kansasii: Implications for epidemiological and pathogenicity studies [J].
Alcaide, F ;
Richter, I ;
Bernasconi, C ;
Springer, B ;
Hagenau, C ;
SchulzeRobbecke, R ;
Tortoli, E ;
Martin, R ;
Bottger, EC ;
Telenti, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (08) :1959-1964
[2]   Soft tissue infections due to non-tuberculous mycobacteria following mesotherapy.: What is the price of beauty? [J].
Alejandra Rivera-Olivero, Ismar ;
Guevara, Armando ;
Escalona, Arnelly ;
Oliver, Margarita ;
Perez-Alfonzo, Ricardo ;
Piquero, Jaime ;
Zerpa, Olga ;
de Waard, Jacobus H. .
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2006, 24 (05) :302-306
[3]   Molecular technique for rapid identification of mycobacteria [J].
AvanissAghajani, E ;
Jones, K ;
Holtzman, A ;
Aronson, T ;
Glover, N ;
Boian, M ;
Froman, S ;
Brunk, CF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (01) :98-102
[4]   PULMONARY INFECTION WITH MYCOBACTERIUM-XENOPI - REVIEW OF TREATMENT AND RESPONSE [J].
BANKS, J ;
HUNTER, AM ;
CAMPBELL, IA ;
JENKINS, PA ;
SMITH, AP .
THORAX, 1984, 39 (05) :376-382
[5]  
Bannalikar AS, 2006, INDIAN J MED RES, V123, P165
[6]   Emergence of Mycobacterium kansasii as the leading mycobacterial pathogen isolated over a 20-year period at a midwestern veterans affairs hospital [J].
Bittner, MJ ;
Horowitz, EA ;
Safranek, TJ ;
Preheim, LC .
CLINICAL INFECTIOUS DISEASES, 1996, 22 (06) :1109-1110
[7]   Incidence and clinical implications of isolation of Mycobacterium kansasii:: Results of a 5-year, population-based study [J].
Bloch, KC ;
Zwerling, L ;
Pletcher, MJ ;
Hahn, JA ;
Gerberding, JL ;
Ostroff, SM ;
Vugia, DJ ;
Reingold, AL .
ANNALS OF INTERNAL MEDICINE, 1998, 129 (09) :698-+
[8]   Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene [J].
Brunello, F ;
Ligozzi, M ;
Cristelli, E ;
Bonora, S ;
Tortoli, E ;
Fontana, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (08) :2799-2806
[9]  
Centers for Disease Control and Prevention (CDC), 2005, MMWR Morb Mortal Wkly Rep, V54, P1127
[10]   Molecular characterization of Mycobacterium kansasii isolates in the state of Sao Paulo between 1995-1998 [J].
Chimara, E ;
Giampaglia, CMS ;
Martins, MC ;
Telles, MAD ;
Ueki, SYM ;
Ferrazoli, L .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2004, 99 (07) :739-743
12345