Preparation of a Functional GABARAP-Lipid Conjugate in Nanodiscs and its Investigation by Solution NMR Spectroscopy

被引：20

作者：

Ma, Peixiang ^{[1
]}

Mohrlueder, Jeannine ^{[1
]}

Schwarten, Melanie ^{[1
,2
]}

Stoldt, Matthias ^{[1
,2
]}

Singh, Sameer K. ^{[1
]}

Hartmann, Rudolf ^{[1
]}

Pacheco, Victor ^{[1
,2
]}

Willbold, Dieter ^{[1
,2
]}

机构：

[1] Res Ctr Julich, Inst Struct Biol & Biophys ISB 3, D-52425 Julich, Germany

[2] Univ Dusseldorf, Inst Phys Biol & BMFZ, D-40225 Dusseldorf, Germany

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 14期

关键词：

membrane proteins; NMR spectroscopy; protein structures; protein-protein interactions; proteolipids; INTEGRAL MEMBRANE-PROTEINS; SUBSTRATE-BINDING; CRYSTAL-STRUCTURE; LIGAND; ATG8; CYTOCHROME-P450; CALRETICULIN; LC3;

D O I：

10.1002/cbic.201000354

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) The state you're in: GABARAP, a protein relevant for autophagic processes, has been enzymatically coupled to phosphatidylethanolamine in a ubiquitin-like modification cascade. The lipidation state of GABARAP defines its subcellular localization pattern and is crucial to its functional integrity. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1967 / 1970

页数：4

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