Chiral separation of 2,4-dinitrophenyl amino acids using 3-O-methyl-β-cyclodextrin-bonded stationary phase in reversed-phase liquid chromatography

被引：12

作者：

Ryu, JW

Kim, DW

Lee, KP

Pyo, D

Park, JH ^{[1
]}

机构：

[1] Yeungnam Univ, Dept Chem, Kyongsan 712749, South Korea

[2] Korea Res Inst Chem Technol, Taejon 305606, South Korea

[3] Kyungpook Natl Univ, Dept Chem Educ, Taegu 702701, South Korea

[4] Kangweon Natl Univ, Dept Chem, Chunchon 200701, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 814卷 / 1-2期

关键词：

chiral stationary phases; LC; enantiomer separation; amino acids; DNP derivatives; cyclodextrins; heptakis(3-O-methyl)-beta-cyclodextrin;

D O I：

10.1016/S0021-9673(98)00410-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversed-phase liquid chromatography (RPLC) separation factors for racemic 2,4-dinitrophenyl (DNP) amino acids (Phe and Trp) having various substituents at different positions were measured on native beta-CD and heptakis(3-O-methyl)-beta-CD as the chiral stationary phase in order to compare enantioselectivity of these phases. Both native beta-CD and heptakis(3-O-methyl)-beta-CD showed good enantioselectivity for the DNP-amino acids investigated. Enantioselectivities of the two CD phases for the DNP-amino acids vary with the type and position of the substituent on the DNP moiety of the amino acids. Heptakis(3-O-methyl)-beta-CD, the cavity of which is more electron-rich than that of native beta-CD, showed in general much better enantioselectivity for the amino acid derivatives studied. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：247 / 252

页数：6

共 17 条

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