Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh platelet-rich plasma

被引：24

作者：

da Silva, Leticia Queiroz ^{[1
]}

de Lima Montalvao, Silmara Aparecida ^{[1
]}

Justo-Junior, Amauri da Silva ^{[2
]}

Rosenberis Cunha Junior, Jose Luiz ^{[1
]}

Huber, Stephany Cares ^{[1
]}

Oliveira, Carolina Caliari ^{[3
]}

Annichino-Bizzacchi, Joyce Maria ^{[1
]}

机构：

[1] State Univ Campinas UNICAMP, Hemoctr, Haemostasis Lab, BR-13083970 Campinas, SP, Brazil

[2] State Univ Campinas UNICAMP, Dept Clin Pathol, Fac Med Sci, BR-13083970 Campinas, SP, Brazil

[3] State Univ Campinas UNICAMP, Fac Med Sci, BR-13083970 Campinas, SP, Brazil

来源：

REGENERATIVE MEDICINE | 2018年 / 13卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

fresh platelet-rich plasma; growth factors; lyophilization; lyophilized platelet-rich plasma; platelet aggregation; platelet-rich plasma; CYTOKINE-RELEASE KINETICS; MESENCHYMAL STEM-CELLS; IN-VITRO; HEALING PROPERTIES; ACTIVATION; PRP; TREHALOSE; MIGRATION; VIVO; GEL;

D O I：

10.2217/rme-2018-0035

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aims: To compare levels and activity of the growth factors between fresh and lyophilized platelet-rich plasma (PRP). Methods: Analysis of platelet concentration using fibroblast and human umbilical vein endothelial cell cultures were compared between fresh and lyophilized PRP obtained from peripheral blood. Results: After lyophilization, 54% of platelets were intact whereas the fresh showed no aggregation with agonists (levels under 20%). The concentration of growth factors (VEGF, EGF, TGF- and PDGF) in both products were similar. Fresh and lyophilized PRPs induced proliferation in the fibroblasts at 24h (0.303 vs 0.300, respectively). Conclusion: Lyophilized PRP appears to be an alternative to fresh PRP and the results evidenced the role of growth factors as a key element in the activity of this product.

引用

页码：775 / 784

页数：10

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