The concentration dependent biphasic effect of leptin on endogenous cholesterol synthesis in human monocytes

被引：9
作者：
Balogh, Zoltan
Foris, Gabriella
Kosztaczky, Bela
Paragh, Gyoergy, Jr.
Seres, Ildiko
Zsiros, Emese
Konya, Gabriella
Paragh, Gyoergy
机构：
[1] Univ Debrecen, DEOEC, Med & Hlth Sci Ctr, Dept Med 1, H-4012 Debrecen, Hungary
[2] Semmelweis Univ, Dept Dermatol, Budapest, Hungary
[3] Univ Debrecen, Med & Hlth Sci Ctr, Dept Biophys & Cell Biol, H-4012 Debrecen, Hungary
来源：
PEPTIDES | 2007年 / 28卷 / 10期
关键词：
D O I：
10.1016/j.peptides.2007.07.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In human monocytes 100 ng/mL leptin increased both statin-inhibitable free radical and cholesterol production in vitro. In our recent study, we aimed to elucidate the concentration dependence of observed leptin-effect. Following leptin stimulation cholesterol synthesis was measured in the presence of inhibitors to determine affected signal pathways. Leptin at low (10-100 ng/mL) concentrations increased [C-14]acetate incorporation, whereas at 250 ng/mL and higher concentrations it suppressed cholesterol synthesis. HMG CoA reductase, phosphatidyl-3-kinase (PI3K) and mitogen activated protein kinase (MAPK) were involved in mediating leptin effects at low concentrations, whereas the cholesterol synthesis suppression was abolished by inhibitors of protein kinase C (PKC) and PI3K. (C) 2007 Published by Elsevier Inc.
引用
收藏
页码：2081 / 2083
页数：3
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