Cloning, DNA sequence analysis, and deletion of a gene encoding diacetyl-acetoin reductase from Lactococcus lactis

被引：6
作者：
Aungpraphapornchai, P [1 ]
Griffin, HG [1 ]
Gasson, MJ [1 ]
机构：
[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England
来源：
DNA SEQUENCE | 1999年 / 10卷 / 03期
关键词：
lactic acid bacteria; flavour; diacetyl; diary products; gene cloning;
D O I：
10.3109/10425179909033942
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Diacetyl is produced by strains of lactic acid bacteria used in the dairy industry. Production of this important flavour compound could be increased by genetic manipulation of genes encoding enzymes involved in diacetyl metabolism. This paper reports the cloning and sequencing of the gene (dar) encoding diacetyl-acetoin reductase from Lactococcus lactis. Analysis of the DNA sequence of the dnr gene and surrounding area revealed the presence of a putative operon with similarity to the family of ABC transporter systems. The nar gene has been deleted from the chromosome by double cross-over homologous recombination.
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页码：163 / 172
页数：10
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