Protein localisation approaches for understanding yeast cell wall biogenesis

被引:0
|
作者
Molina, M [1 ]
Gil, C [1 ]
Pla, J [1 ]
Arroyo, J [1 ]
Nombela, C [1 ]
机构
[1] Univ Complutense Madrid, Fac Farm, Dept Microbiol 2, E-28040 Madrid, Spain
基金
中国国家自然科学基金;
关键词
mannoprotein; epitope-tagging; GFP; proteomics; GPI;
D O I
10.1002/1097-0029(20001215)51:6<601::AID-JEMT9>3.0.CO;2-I
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Yeast cells are surrounded by the cell wall, a rigid but dynamic structure that is essential for their viability. The complexity and functionality of this structure suggest that a high number of proteins must be involved in the biogenesis of the cell wall architecture and, as a consequence, in the maintenance of cell integrity. Among them, a high percentage is assumed to be located at the cell surface, mostly as structural or enzymatic components of the cell wall. Therefore, the presence of a protein in the cell wall is suggestive of its cell wall-related function. Different techniques can be used to specifically detect the cell wall localisation of a given protein or to identify cell wall proteins in large-scale analyses. These include the detection of proteins in whole cells or specific cell wall fractions by immunological, biochemical, microscopic, or genetic approaches, as well as the emerging proteomic technology. The advantages, limitations, and usefulness of these techniques are discussed and illustrated with some examples. (C) 2000 Wiley-Liss, Inc.
引用
收藏
页码:601 / 612
页数:12
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