Dynamic-Light-Scattering-Based Sequence-Specific Recognition of Double-Stranded DNA with Oligonucleotide-Functionalized Gold Nanoparticles

被引：43
作者：
Miao, Xiang-Min [1 ,2 ]
Xiong, Cen [1 ]
Wang, Wei-Wei [1 ,2 ]
Ling, Lian-Sheng [1 ]
Shuai, Xin-Tao [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Zhongshan Sch Med, BME Ctr, Guangzhou 510275, Guangdong, Peoples R China
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 40期
基金：
中国国家自然科学基金;
关键词：
DNA recognition; gold; oligonucleotides; nanoparticles; triplex DNA; TRIPLEX-FORMING OLIGONUCLEOTIDES; PEPTIDE NUCLEIC-ACIDS; DUPLEX DNA; PROBES; INHIBITION; POLYAMIDES;
D O I：
10.1002/chem.201003010
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An ultrasensitive and simple dynamic-light-scattering (DLS) assay for the sequence-specific recognition of double-stranded DNA (dsDNA) was developed based on detection of the average diameter change of Au nanoparticle (AuNP) probes modified with oligonucleotides 5'-TTTCTCTTCCTTCTCTTC-(T)(12)-SH-3' (Oligo 1) and 5'-TTCTTTCTTTTCTTTTTC-(T)(12)-SH-3' (Oligo 2). The target dsDNA was composed of two complementary oligo-nucleotides: 5'-AAAGAGAAGGAAGAGAAGAAGAAAGAAAAGAAAAAG-3' (Oligo 3) and 3'-TTTCTCTTCCTTCTCTTCTTCTTTCTTTTCTTTTTC-5' (Oligo 4). Hybridization of the two AuNPs-Oligo probes with the target dsDNA induced aggregation of the target dsDNA by forming triplex DNA, which accordingly increased the average diameter. This diameter change could then be detected by DLS. The average diameter was proportional to the target dsDNA concentration over the range from 593 fM to 40 pm, with a detection limit of 593 fM. Moreover, the assay had good sequence specificity for the target dsDNA.
引用
收藏
页码：11230 / 11236
页数：7
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