Transcriptomic Analysis of Haematococcus lacustris during Astaxanthin Accumulation under High Irradiance and Nutrient Starvation

被引：35
作者：
Kim, Dong-Keon [1 ]
Hong, Seong-Joo [2 ]
Bae, Jae-Han [2 ]
Yim, Narae [2 ]
Jin, EonSeon [3 ]
Lee, Choul-Gyun [1 ,2 ]
机构：
[1] Inha Univ, Dept Energy Engn, Inchon 402751, South Korea
[2] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea
[3] Hanyang Univ, Dept Life Sci, Seoul 133791, South Korea
来源：
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2011年 / 16卷 / 04期
关键词：
Haematococcus lacustris; astaxanthin; cDNA microarray; transcriptomics; high irradiance; nutrient starvation; ISOPRENOID BIOSYNTHESIS; ESCHERICHIA-COLI; PLUVIALIS; EXPRESSION; ALGA; STRESS; PATHWAY; PROTEIN; CHLOROPHYCEAE; DIPHOSPHATE;
D O I：
10.1007/s12257-011-0081-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this study, a cDNA microarray was developed from over 60,000 mRNA readings to analyze the expression profiles of transcriptomes of Haematococcus lacustris under astaxanthin-inducing culture conditions, high irradiance and nitrate starvation. Among 20,033 genes on the cDNA microarray, 2,675 genes exhibited a twofold or greater difference in expression. Of these, 1,333 genes were up-regulated and 1,342 genes were down-regulated. A significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were observed under astaxanthin inducing conditions (forming red cyst cells). On the other hand, respiration-related genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells under stress conditions. These results enabled a better understanding of the cell responses during stress induction of H. lacustris such as photosynthesis, respiration, and some biopathways.
引用
收藏
页码：698 / 705
页数：8
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