Lentivirus-mediated overexpression of TGF-β inducible early gene 1 inhibits SW1990 pancreatic cancer cell growth

被引：5

作者：

Jiang, Lei ^{[1
]}

Wang, Fule ^{[1
]}

Lin, Feiyan ^{[1
]}

Gao, Shen-Meng ^{[1
]}

Tan, Yingxia ^{[1
]}

Han, Yixiang ^{[1
]}

Chen, Chiqi ^{[1
]}

Wu, Jianbo ^{[1
]}

机构：

[1] Wenzhou Med Coll, Affiliated Hosp 1, Lab Internal Med, Wenzhou 325000, Zhejiang, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2011年 / 35卷 / 09期

关键词：

lentivirus; pancreatic cancer; TIEG1; tumourigenicity; TRANSCRIPTION FACTOR; TIEG; APOPTOSIS; IDENTIFICATION; EXPRESSION; THERAPY; TISSUE;

D O I：

10.1042/CBI20100896

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

TIEG1 (TGF-beta inducible early gene 1) plays a significant role in regulating cell proliferation and apoptosis in various cell types. Previous studies have shown a close relationship between the expression level of TIEG1 and various cancers, including breast, prostate, colorectal and pancreatic cancer. In this study, we up-regulated the gene expression of TIEG1 in SW1990 pancreatic cancer cell line by a lentivirus transfection system and investigated its potential as a therapeutic target for pancreatic cancer. The results showed that lentivirus-mediated overexpression of TIEG1 gene inhibited human pancreatic cancer SW1990 cell proliferation and caused the cell cycle arrest at the G(1)-phase in vitro. SW1990 cells transduced with lenti-TIEG1 showed significant inhibition of colony formation and cancer cell growth in 3-D culture model. Moreover, overexpression of TIEG1 gene significantly slowed the growth of SW1990 xenografts in nude mice. Taken together, these data provided evidence that overexpression of TIEG1 gene by a lentivirus transfection system led to suppressed human pancreatic cancer cell growth and might therefore be a feasible approach in the clinical management of pancreatic cancer.

引用

页码：891 / 896

页数：6

共 29 条

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