Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

被引：23
作者：
Gajaria, Tejal K. [1 ]
Patel, Dipak K. [1 ]
Devkar, Ranjitsinh V. [1 ]
Ramachandran, A. V. [1 ]
机构：
[1] Maharaja Sayaji Rao Univ Baroda, Fac Sci, Dept Zool, Div Phytotherapeut & Metab Endocrinol, Vadodara 390002, India
来源：
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE | 2015年 / 52卷 / 06期
关键词：
Ox-LDL; Atherosclerosis; RAW 264.7 macrophage cells; Oxidative stress; Murraya koenigii; LOW-DENSITY-LIPOPROTEIN; ANTIOXIDANT ACTIVITY; MITOCHONDRIAL DYSFUNCTION; L; LEAVES; POLYPHENOLS; GROWTH; ASSAY;
D O I：
10.1007/s13197-014-1399-2
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Several lines of evidences have established a lineage between Oxidised LDL (Ox-LDL) to apoptosis of macrophages in which the high level of intracellular cholesterol play a crucial role. This study assesses the potency of Murraya koenigii (MK) leaf extract in alleviating LDL oxidation and Ox-LDL induced lipotoxicity in murine macrophage (RAW 264.7) cells. Results indicated that presence of MK extract prevented oxidation of LDL as evidenced by its oxidation kinetics and formation of LDL oxidation products. Also, MK extract accounted for improvement in cell viability and mitochondrial membrane potential of Ox-LDL treated cells. The Ox-LDL induced increment in intracellular oxidative stress, nuclear condensation and apoptosis was effectively prevented by MK extract possibly due to their established anti-oxidant and free radical scavenging potentials which may be attributed to the presence of flavonoids present in the extract. Prevention of oxidative modification of LDL, free radical induced damage and Ox-LDL induced death of RAW 264.7 cells provide preliminary evidences of its anti-atherosclerotic potential and warrants further elucidation and validation for its use in-vivo and may be useful as a functional food supplement and an alternative medicine to prevent LDL oxidation and oxidized LDL induced toxicity.
引用
收藏
页码：3367 / 3375
页数：9
相关论文
共 43 条
[11]  
Degli EM, 2001, METHOD CELL BIOL, V65, P75, DOI DOI 10.1016/S0091-679X(01)65005-2
[12]   Hepatoprotective potential of polyphenol rich extract of Murraya koenigii L.: An in vivo study [J].
Desai, Swati N. ;
Patel, Dipak K. ;
Devkar, Ranjitsinh V. ;
Patel, Prabhudas V. ;
Ramachandran, A. V. .
FOOD AND CHEMICAL TOXICOLOGY, 2012, 50 (02) :310-314
[13]   CONTINUOUS MONITORING OF INVITRO OXIDATION OF HUMAN LOW-DENSITY LIPOPROTEIN [J].
ESTERBAUER, H ;
STRIEGL, G ;
PUHL, H ;
ROTHENEDER, M .
FREE RADICAL RESEARCH COMMUNICATIONS, 1989, 6 (01) :67-75
[14]   MTT COLORIMETRIC ASSAY FOR TESTING MACROPHAGE CYTOTOXIC ACTIVITY INVITRO [J].
FERRARI, M ;
FORNASIERO, MC ;
ISETTA, AM .
JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) :165-172
[15]  
González-Juanatey JR, 2004, J ENDOCRINOL, V180, P325
[16]   Oxidants and antioxidants in the pathogenesis of atherosclerosis: implications for the oxidized low density lipoprotein hypothesis [J].
Heinecke, JW .
ATHEROSCLEROSIS, 1998, 141 (01) :1-15
[17]   Extract from the leaf of nucifera reduced the development of atherosclerosis via inhibition of vascular smooth muscle cell proliferation and migration [J].
Ho, Hsieh-Hsun ;
Hsu, Li-Sung ;
Chan, Kuei-Chuan ;
Chen, Hsiang-Mei ;
Wu, Cheng-Hsun ;
Wang, Chau-Jong .
FOOD AND CHEMICAL TOXICOLOGY, 2010, 48 (01) :159-168
[18]   Targeting mitochondria [J].
Hoye, Adam T. ;
Davoren, Jennifer E. ;
Wipf, Peter ;
Fink, Mitchell P. ;
Kagan, Valerian E. .
ACCOUNTS OF CHEMICAL RESEARCH, 2008, 41 (01) :87-97
[19]   Standardized Flavonoid-Rich Eugenia jambolana Seed Extract Retards In Vitro and In Vivo LDL Oxidation and Expression of VCAM-1 and P-Selectin in Atherogenic Rats [J].
Jadeja, Ravirajsinh N. ;
Thouaojam, Menaka C. ;
Sankhari, Jayantha M. ;
Jain, Mahendra ;
Devkar, Ranjitsinh V. ;
Ramachandran, A. V. .
CARDIOVASCULAR TOXICOLOGY, 2012, 12 (01) :73-82
[20]   Clerodendron glandulosum.Coleb extract prevents in vitro human LDL oxidation and oxidized LDL induced apoptosis in human monocyte derived macrophages [J].
Jadeja, Ravirajsinh N. ;
Thounaojam, Menaka C. ;
Devkar, Ranjisinh V. ;
Ramachandran, A. V. .
FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (06) :1195-1202
12345