Gibberellin-Induced Alterations to the Expression of Cell Wall-Related Genes in the Xylem of Carrot Root

Gibberellins (GAs) are a group of plant hormones that play important roles in various processes. Previous studies demonstrated that GA can increase the cellulose content and lignification of plant tissues. However, the effects of altered GA levels on carrot root development have not been fully characterized. In this study, carrot roots were treated with exogenous GA(3), after which thickened cell walls were detected in the xylem area. Transcriptome sequencing data revealed the changes induced by GA(3). A total of 5416 differentially expressed genes (DEGs) were identified, of which 3011 and 2405 were up- and down-regulated, respectively. The DEGs were enriched in metabolic pathways, with 236 DEGs related to the cell wall. Digital gene expression data and the verification by qRT-PCR indicated that exogenous GA can alter the expression of genes involved in cell wall synthesis, suggesting a potential role for GA in cell wall organization and modification. The results of this study provide novel insights into the GA functions influencing carrot root development.