Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics

被引：13
|
作者：
Zhu, Kongyang [1 ]
Du, Panxin [2 ]
Xiong, Jianxue [2 ]
Ren, Xiaoying [3 ]
Sun, Chang [2 ]
Tao, Yichen [2 ]
Ding, Yi [3 ]
Xu, Yiran [2 ]
Meng, Hailiang [2 ]
Wang, Chuan-Chao [1 ]
Wen, Shao-Qing [2 ,3 ]
机构：
[1] Xiamen Univ, Sch Life Sci,Dept Anthropol & Ethnol, Sch Sociol & Anthropol,State Key Lab Marine Envir, Inst Anthropol,State Key Lab Cellular Stress Biol, Xiamen, Peoples R China
[2] Fudan Univ, Sch Life Sci, Dept Anthropol & Human Genet, MOE Key Lab Contemporary Anthropol, Shanghai, Peoples R China
[3] Fudan Univ, Inst Archaeol Sci, Shanghai, Peoples R China
来源：
FRONTIERS IN GENETICS | 2021年 / 12卷
基金：
欧洲研究理事会; 国家重点研发计划; 中国国家自然科学基金;
关键词：
MGISEQ-2000; BGI tech; ancient DNA; paleogenomics; population genetics; ANCIENT DNA; POPULATION-STRUCTURE; FRAMEWORK; EXTRACTION; EFFICIENT; ADMIXTURE;
D O I：
10.3389/fgene.2021.745508
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The MGISEQ-2000 sequencer is widely used in various omics studies, but the performance of this platform for paleogenomics has not been evaluated. We here compare the performance of MGISEQ-2000 with the Illumina X-Ten on ancient human DNA using four samples from 1750BCE to 60CE. We found there were only slight differences between the two platforms in most parameters (duplication rate, sequencing bias, theta, delta S, and lambda). MGISEQ-2000 performed well on endogenous rate and library complexity although X-Ten had a higher average base quality and lower error rate. Our results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEQ-2000 can be an alternative platform for paleogenomics sequencing.</p>
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页数：9
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