Curcumin inhibits alloxan-induced pancreatic islet cell damage via antioxidation and antiapoptosis

被引：13

作者：

Xia, Zhen-hong ^{[1
]}

Jiang, Xue ^{[1
]}

Li, Ke ^{[1
]}

Li, Li-xia ^{[1
]}

Chen, Wen-bo ^{[1
]}

Wang, Yu-xiang ^{[1
]}

Liu, Yan-qiang ^{[1
]}

机构：

[1] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2020年 / 34卷 / 07期

基金：

中国国家自然科学基金;

关键词：

alloxan; apoptosis; curcumin; RIN-m5F cell; DIABETES-MELLITUS; OXIDATIVE STRESS; MECHANISM;

D O I：

10.1002/jbt.22499

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study elucidates the possible protective effects of curcumin on beta-cells damaged by oxidative stress and its significance in controlling diabetes mellitus in in vitro experiments. Pancreatic islet (RIN-m5F) cells were treated with 25 mmol/L alloxan (AXN) to induce cell damage and the protective effects of curcumin were observed. The results showed that curcumin significantly promoted the cellular activity of AXN-treated RIN-m5F cells, decreased the ratio of apoptosis, downregulated the level of malondialdehyde, upregulated the levels of superoxide dismutase and reactive oxygen species, increased the expression of Bcl-2, cleaved caspase-3, and cleaved PARP1, and decreased the expression of Bax in AXN-treated cells. These results suggest that curcumin inhibits AXN-induced damage in RIN-m5F cells via antioxidative and antiapoptotic mechanisms.

引用

页数：9

共 35 条

[21] Protective role of arjunolic acid in response to streptozotocin-induced type-I diabetes via the mitochondrial dependent and independent pathways [J].

Manna, Prasenjit ;

Sinha, Mahua ;

Sil, Parames C. .

TOXICOLOGY, 2009, 257 (1-2) :53-63

[22] Chemical studies on antioxidant mechanism of curcuminoid: Analysis of radical reaction products from curcumin [J].

Masuda, T ;

Hidaka, K ;

Shinohara, A ;

Maekawa, T ;

Takeda, Y ;

Yamaguchi, H .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (01) :71-77

[23] Protective effect of curcumin against seizures and cognitive impairment in a pentylenetetrazole-kindled epileptic rat model [J].

Mehla, Jogender ;

Reeta, K. H. ;

Gupta, Pooja ;

Gupta, Yogendra Kumar .

LIFE SCIENCES, 2010, 87 (19-22) :596-603

[24] Nutrient regulation of insulin secretion and action [J].

Newsholme, Philip ;

Cruzat, Vinicius ;

Arfuso, Frank ;

Keane, Kevin .

JOURNAL OF ENDOCRINOLOGY, 2014, 221 (03) :R105-R120

[25] FREE-RADICALS AND DIABETES [J].

OBERLEY, LW .

FREE RADICAL BIOLOGY AND MEDICINE, 1988, 5 (02) :113-124

[26]

Pavana P, 2007, J ENVIRON BIOL, V28, P833

[27] Curcumin protects against hepatic stellate cells activation and migration by inhibiting the CXCL12/CXCR4 biological axis in liver fibrosis: A study in vitro and in vivo [J].

Qin, Lifeng ;

Qin, Jinmei ;

Zhen, Xiumei ;

Yang, Qian ;

Huang, Liyi .

BIOMEDICINE & PHARMACOTHERAPY, 2018, 101 :599-607

[28] Type 2 diabetes -: A matter of β-cell life and death? [J].

Rhodes, CJ .

SCIENCE, 2005, 307 (5708) :380-384

[29] Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property [J].

Sabu, MC ;

Kuttan, R .

JOURNAL OF ETHNOPHARMACOLOGY, 2002, 81 (02) :155-160

[30] Diabetes, oxidative stress, molecular mechanism, and cardiovascular disease - an overview [J].

Selvaraju, Vaithinathan ;

Joshi, Mandip ;

Suresh, Sumanth ;

Sanchez, Juan A. ;

Maulik, Nilanjana ;

Maulik, Gautam .

TOXICOLOGY MECHANISMS AND METHODS, 2012, 22 (05) :330-335

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