Curcumin inhibits alloxan-induced pancreatic islet cell damage via antioxidation and antiapoptosis

被引：13
作者：
Xia, Zhen-hong [1 ]
Jiang, Xue [1 ]
Li, Ke [1 ]
Li, Li-xia [1 ]
Chen, Wen-bo [1 ]
Wang, Yu-xiang [1 ]
Liu, Yan-qiang [1 ]
机构：
[1] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
来源：
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2020年 / 34卷 / 07期
基金：
中国国家自然科学基金;
关键词：
alloxan; apoptosis; curcumin; RIN-m5F cell; DIABETES-MELLITUS; OXIDATIVE STRESS; MECHANISM;
D O I：
10.1002/jbt.22499
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study elucidates the possible protective effects of curcumin on beta-cells damaged by oxidative stress and its significance in controlling diabetes mellitus in in vitro experiments. Pancreatic islet (RIN-m5F) cells were treated with 25 mmol/L alloxan (AXN) to induce cell damage and the protective effects of curcumin were observed. The results showed that curcumin significantly promoted the cellular activity of AXN-treated RIN-m5F cells, decreased the ratio of apoptosis, downregulated the level of malondialdehyde, upregulated the levels of superoxide dismutase and reactive oxygen species, increased the expression of Bcl-2, cleaved caspase-3, and cleaved PARP1, and decreased the expression of Bax in AXN-treated cells. These results suggest that curcumin inhibits AXN-induced damage in RIN-m5F cells via antioxidative and antiapoptotic mechanisms.
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页数：9
相关论文
共 35 条
[1]   ROLE OF OXIDATIVE STRESS IN DEVELOPMENT OF COMPLICATIONS IN DIABETES [J].
BAYNES, JW .
DIABETES, 1991, 40 (04) :405-412
[2]  
BENNETT RA, 1981, CANCER RES, V41, P2786
[3]   β-cell deficit and increased β-cell apoptosis in humans with type 2 diabetes [J].
Butler, AE ;
Janson, J ;
Bonner-Weir, S ;
Ritzel, R ;
Rizza, RA ;
Butler, PC .
DIABETES, 2003, 52 (01) :102-110
[4]   Antiviral activity of silymarin against Mayaro virus and protective effect in virus-induced oxidative stress [J].
Camini, Fernanda Caetano ;
da Silva, Tales Fernando ;
da Silva Caetano, Camila Carla ;
Almeida, Leticia Trindade ;
Ferraz, Ariane Coelho ;
Alves Vitoreti, Veronica Maria ;
Silva, Breno de Mello ;
Silva, Silvana de Queiroz ;
de Magalhaes, Jose Carlos ;
de Brito Magalhaes, Cintia Lopes .
ANTIVIRAL RESEARCH, 2018, 158 :8-12
[5]   Preservation of β-cell function by targeting β-cell mass [J].
de Koning, Eelco J. P. ;
Bonner-Weir, Susan ;
Rabelink, Ton J. .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2008, 29 (04) :218-227
[6]   The role of uncoupling protein 2 (UCP2) on the development of type 2 diabetes mellitus and its chronic complications [J].
de Souza, Bianca Marmontel ;
Assmann, Tais Silveira ;
Kliemann, Lucia Maria ;
Gross, Jorge Luiz ;
Canani, Luis Henrique ;
Crispim, Daisy .
ARQUIVOS BRASILEIROS DE ENDOCRINOLOGIA E METABOLOGIA, 2011, 55 (04) :239-248
[7]   The antihyperglycemic effect of curcumin in high fat diet fed rats. Role of TNF-α and free fatty acids [J].
El-Moselhy, Mohamed A. ;
Taye, Ashraf ;
Sharkawi, Sara Shaaban ;
El-Sisi, Suzan F. I. ;
Ahmed, Ahmed Fahmy .
FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (05) :1129-1140
[8]   The use of vitamin E in type 2 diabetes mellitus [J].
Fardoun, Riham Zein .
CLINICAL AND EXPERIMENTAL HYPERTENSION, 2007, 29 (03) :135-148
[9]   Differential effects of curcumin on vasoactive factors in the diabetic rat heart [J].
Farhangkhoee, Hana ;
Khan, Zia A. ;
Chen, Shali ;
Chakrabarti, Subrata .
NUTRITION & METABOLISM, 2006, 3 (1)
[10]   Curcumin labels amyloid pathology in vivo, disrupts existing plaques, and partially restores distorted neurites in an Alzheimer mouse model [J].
Garcia-Alloza, M. ;
Borrelli, L. A. ;
Rozkalne, A. ;
Hyman, B. T. ;
Bacskai, B. J. .
JOURNAL OF NEUROCHEMISTRY, 2007, 102 (04) :1095-1104
1234