Ionic liquid facilitates biocatalytic conversion of hardly water soluble ketones

被引：28

作者：

Kohlmann, Christina ^{[2
]}

Robertz, Nora ^{[2
]}

Leuchs, Susanne ^{[1
,2
]}

Dogan, Zuebeyde ^{[2
]}

Luetz, Stephan ^{[3
]}

Bitzer, Katrin ^{[4
]}

Na'amnieh, Shukrallah ^{[4
]}

Greiner, Lasse ^{[1
,2
]}

机构：

[1] DECHEMA eV, Karl Winnacker Inst, D-60486 Frankfurt, Germany

[2] Rhein Westfal TH Aachen, Inst Tech & Makromol Chem, D-52074 Aachen, Germany

[3] Forschungszentrum Julich GmbH, Inst Biotechnol 2, D-52425 Julich, Germany

[4] X Zyme GmbH, D-40225 Dusseldorf, Germany

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2011年 / 68卷 / 02期

关键词：

Ionic liquids; Biotransformations; Chiral alcohols; Performance additive; Enantioselective reduction; Alcohol dehydrogenase; ENZYME CATALYSIS; PERFORMANCE ADDITIVES; ALCOHOL-DEHYDROGENASE; BIOTRANSFORMATIONS; REDUCTION; REGENERATION; SOLVENTS; SYSTEMS;

D O I：

10.1016/j.molcatb.2010.10.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ionic liquids represent a promising alternative to conventional cosolvents as biocompatible solubilisers for biocatalysis. This was shown using water miscible ionic liquids to facilitate the stereoselective reduction of hardly water soluble, aliphatic ketones catalysed by the alcohol dehydrogenase from Lactobacillus brevis. Ten ionic liquids were screened for activity and solubility. Improved storage stabilities besides improved enzyme activities, as well as reduced substrate surplus and product inhibitions were found, while applying the most promising AMMOENG (TM) 101 in more detailed investigations. Batch reactions with cofactor regeneration via a glucose dehydrogenase showed increased reaction rates; thus underlining the positive influence of AMMOENG (TM) 101. For (R)-3-octanol, (R)-2-nonanol, (R)-2-decanol, and (R)-2-octanol space time yields between 250 and 350 mmol L-1 d(-1) were achieved. (c) 2010 Elsevier B.V. All rights reserved.

引用

页码：147 / 153

页数：7

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