Optimizing extraction conditions of crude fiber, phenolic compounds, flavonoids and antioxidant activity of date seed powder

被引:15
作者
Afifi, Hanan S. [1 ,2 ]
Hashim, Isameldin B. [1 ]
Altubji, Sabreen I. [1 ]
机构
[1] United Arab Emirates Univ, Food Sci Dept, Coll Food & Agr, POB 17551, Al Ain, U Arab Emirates
[2] Abu Dhabi Food Control Author, R&D Div, POB 52150, Abu Dhabi, U Arab Emirates
来源
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE | 2017年 / 54卷 / 13期
关键词
Response surface methodology; Date seeds powder; Crude fiber; Phenolic compounds; Flavonoids; Antioxidant activity; DIETARY FIBER; OPTIMIZATION; POLYPHENOLS; STABILITY; SOLVENTS; FRUIT; FOOD;
D O I
10.1007/s13197-017-2854-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The objective of this study was to optimize the extraction conditions of crude fiber, phenolic compounds, flavonoids, and antioxidant activity from date seeds powder, using Response Surface Methodology. A central composite design with four independent variables; concentration of ethanol (X-1 = 25, 50 and 75% v/v), solvent: sample ratio (X-2 = 40:1, 50:1 and 60:1 v/w), temperature (X-3 = 45, 55 and 65 A degrees C), and extraction time (X-4 = 1, 2 and 3 h) and a three level face centered cube design were used. A total of twenty nine experimental runs with five replicates at the central point were used to study the response variables using two extraction cycles. Maximum phenolic compound content (71.6 mg GAE/100 g) was extracted using 50% ethanol solution with 40:1 solvent: sample ratio for 1 h at 55 A degrees C. While the maximum antioxidant activity (55.02 A mu mol Fe(II)/g) was obtained using similar ethanol concentration and solvent: sample ratio except at lower temperature (45 A degrees C) for 2 h. On other hand, the maximum flavonoids content (455.77 mg CEQ/100 g) was reached by using 50% concentration, 50:1 solvent: sample ratio at 65 A degrees C for 3 h. In contrast, the content of fiber was not affected by the different extraction conditions. Results indicate that using combination of extracted conditions, have a great potential for extracting all depending compounds except crude fiber.
引用
收藏
页码:4149 / 4161
页数:13
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