Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/β-catenin signaling pathways

被引：40

作者：

Ghanavati, Roya ^{[1
,2
]}

Asadollahi, Parisa ^{[2
,3
]}

Shapourabadi, Mina Bahrololomi ^{[4
]}

Razavi, Shabnam ^{[2
]}

Talebi, Malihe ^{[1
,2
]}

Rohani, Mahdi ^{[5
]}

机构：

[1] Iran Univ Med Sci, Microbial Biotechnol Res Ctr, Tehran, Iran

[2] Iran Univ Med Sci, Sch Med, Dept Microbiol, Tehran, Iran

[3] Ilam Univ Med Sci, Fac Med, Microbiol Dept, Ilam, Iran

[4] Pasteur Inst Iran, Dept Virol, Tehran, Iran

[5] Pasteur Inst Iran, Dept Microbiol, Tehran, Iran

来源：

MICROBIAL PATHOGENESIS | 2020年 / 139卷

关键词：

Lactobacilli; Anti-proliferation; Apoptosis; Notch; Wnt/beta-catenin; NF-KAPPA-B; PROBIOTICS; APOPTOSIS; MICROBIOTA; BACTERIA; TARGET; ROLES; WNT;

D O I：

10.1016/j.micpath.2019.103829

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Probiotics could be considered as attractive candidates for preventing tumor growth through maintaining homeostasis. The aim of this study was to evaluate the inhibitory effect of a cocktail of five Lactobacillus species on human colorectal carcinoma cell line HT-29. The anti-proliferative and apoptotic effects of Lactobacilli cocktail were evaluated using MTT and flow cytometry tests, respectively. Quantitative real-time polymerase chain reaction (qPCR) was used to analyze the expression of several genes in the Notch (notch, hes1, msi1, and numb) and Wnt/beta-catenin (CTNNB1 and CCND1) pathways, following the treatment of HT-29 cells with Lactobacilli cocktail. The treatment by Lactobacilli cocktail induced a significant anti-proliferative effect and late stage apoptosis among the cancer cells (p < 0.05). Compared to the untreated cells, Lactobacilli cocktail induced the down-regulation of notch, hest, and msi1 genes and up-regulation of numb gene in the Notch pathway as well as the down-regulation of CTNNB1 and CCND1 genes in the Wnt/beta-catenin pathway in a time-dependent manner (p < 0.05). Conclusion: Lactobacilli cocktail was shown to have beneficial anti-tumor effects on HT-29 cells by modulating the Notch and Wnt/beta-catenin pathways; therefore, the use of Lactobacilli probiotics as nutritional supplements may prevent the incidence of colon cancer.

引用

页数：9

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