Anti-hemorrhagic activity of ethanol extract of Moringa oleifera leaf on envenomed albino rats

被引：9

作者：

Adeyemi, Sodiq ^{[1
]}

Larayetan, Rotimi ^{[2
,3
]}

Onoja, A. D. ^{[2
]}

Ajayi, Abayomi ^{[4
]}

Yahaya, Abdulrazaq ^{[2
,3
]}

Ogunmola, Oluranti O. ^{[5
]}

Adeyi, A. O. ^{[6
]}

Chijioke, Olisah ^{[7
]}

机构：

[1] EACOED, Sch Sci, Dept Biol, Oyo, Oyo State, Nigeria

[2] Kogi State Univ, Dept Chem, Anyigba, Nigeria

[3] Univ Ft Hare, Dept Pure & Appl Chem, ZA-5700 Alice, South Africa

[4] Kogi State Univ, Coll Hlth Sci, Fac Basic Med Sci, Dept Anat, Anyigba, Anyigba Kogi St, Nigeria

[5] Emmanuel Alayande Coll Educ, Dept Chem, Oyo, Oyo State, Nigeria

[6] Univ Ibadan, Dept Zool, Anim Physiol Unit, ZA-6031 Port Elizabeth, South Africa

[7] Nelson Mandela Univ, Inst Coastal & Marine Res, Bot Dept, ZA-6031 Port Elizabeth, South Africa

来源：

SCIENTIFIC AFRICAN | 2021年 / 12卷

关键词：

Echis ocellatus; Moringa oleifera; Haemorrhage; Anti-hemorrhagic; Antivenom; Bioactive components; GC-MS analysisC-MS analysis; ROOT EXTRACT; VENOM; NEUTRALIZATION; SNAKEBITES; INHIBITOR;

D O I：

10.1016/j.sciaf.2021.e00742

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Echis ocellatus (E. ocellatus) is one of the most venomous viper species which constitutes significant economic and health problem all over the world, especially in rural African communities. Moringa oleifera (M. oleifera) is a tropical plant traditionally used in Nigeria for the treatment of many diseases including snakebites. This study, therefore, investigated the anti-haemorrhagic property of ethanol extract of M. oleifera leaf on E. ocellatus envenomed male albino rats. A comprehensive account of the bioactive compounds present in the M. oleifera ethanol leaf extract by means of using Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed the presence of 9, 12, 15-Octadecatrienoic acid (39.66%), hexadecanoic acid (16.71%), palmitic acid, ethyl ester (14.70%) and phytol (9.81%) as the major components of the leaf extract and some of these components serves as inhibitors of neurotoxins by suitably binding to the snake venom's toxic protein thereby terminating the poisonous effect of the venom. The LD50 of crude E. ocellatus venom was determined to be 0.22 mg/kg while that of ethanol extract of M. oleifera leaf was 800 mg/kg. Envenomation of E. ocellatus venom results in deleterious hematological and histopathological changes in the heart in a dose-dependent manner. Rat given 0.22 mg/kg of E. ocellatus venom (2 mL) and treated with 200 mg/kg, 400 mg/kg and 800 mg/kg of M. oleifera ethanolic leaf extract (2 ml) intradermally show the least heamorrhagic foci at 800 mg/kg of the extract. Pre-incubation of the extract with E. ocellatus venom before injecting animals further enhances the anti-haemorrhagic action of M. oleifera ethanolic leaf extract. Our findings revealed that M. oleifera ethanolic leaf extract is a promising therapeutic agent for the treatment of E. ocellatus snakebite envenomation. Hence, further studies on M. oleifera leaf are warranted. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of African Institute of Mathematical Sciences / Next Einstein Initiative.

引用

页数：10

共 43 条

[1] Ethnomedical treatment of poisonous snakebites:: Plant extract neutralized Naja nigricollis venom [J].