Optimal handling of fresh cancellous bone graft -: Different peroperative storing techniques evaluated by in vitro osteoblast-like cell metabolism

We investigated the influence of three peroperative handling techniques on the quality of autogenous bone graft by means of osteoblast-like cell metabolism in vitro. Cancellous bone was harvested from the iliac crest of 12 4-month-old female pigs. Osteoblast-like cell cultures were established, using the tissue-explant method: (1) immediately after harvest of bone, (2) after storage of bone in saline at room temperature for 2 hours and (3) after "dry" storage of bone at room temperature for 2 hours. Proliferation was assessed by H-3-thymidine incorporation. Differentiation was assessed by alkaline phosphatase activity and procollagen I production (PICP). We found that osteoblast-like-cell proliferation was higher, when cultures were started shortly after harvesting of bone, or else stored in saline for 2 hours, as compared to bone left "to dry" for 2 hours. Basal alkaline phosphatase and PICP production did not differ in the three groups. These in vitro results suggest the superiority of harvest of autogenous bone graft shortly before the grafting procedure, or else temporary storage of the graft in saline for up to 2 hours.