A microdialysis fibre based sampler for flow injection analysis: Determination of L-lactate in biofluids by an electrochemically synthesised bilayer membrane based biosensor

被引：39

作者：

Palmisano, F ^{[1
]}

Centonze, D ^{[1
]}

Quinto, M ^{[1
]}

Zambonin, PG ^{[1
]}

机构：

[1] UNIV BARI,DIPARTMENTO CHIM,I-70126 BARI,ITALY

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 04期

关键词：

L-lactate; microdialysis; biosensors; flow injection analysis;

D O I：

10.1016/0956-5663(96)82737-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A microdialysis fibre based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is required. This device used in combination with a lactate amperometric biosensor based on lactate oxidase electrochemically immobilised in a bilayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole) permits the extension of the linear range of response up to 10 mM lactate. Combining microdialysis sampling with FIA and amperometric detection at an interference-free and fast-response biosensor, lactate determination in complex media such as serum, milk and yoghurt can be easily achieved with a high sample throughput and no sample pre-treatment.

引用

页码：419 / 425

页数：7

共 17 条

[11] AN IN-SITU ELECTROSYNTHESIZED AMPEROMETRIC BIOSENSOR BASED ON LACTATE OXIDASE IMMOBILIZED IN A POLY-O-PHENYLENEDIAMINE FILM - DETERMINATION OF LACTATE IN SERUM BY FLOW-INJECTION ANALYSIS [J].